伴放线放线杆菌血清型分析  

Identification of serotypes of Actinobacillus actinomycetemcomitans by multiplex PCR

作　　者：刘冬宇[1] 王者玲[2] 王明荣[3] 韩亚玲[3] 杨圣辉[2] 

机构地区：[1]首都医科大学附属北京安贞医院口腔科,北京100029 [2]首都医科大学附属北京口腔医院 [3]中国医学科学院肿瘤研究所

出　　处：《现代口腔医学杂志》2007年第3期271-274,277,共5页Journal of Modern Stomatology

基　　金：北京自然科学基金项目(70520);首都医学发展基金项目(20023021)

摘　　要：目的PCR法检测伴放线放线杆菌(Actinobacillus actinomycetemcomitans,Aa)临床分离菌株血清型,分析其与flp-1基因型的关系。方法用血清型特异性引物,通过普通PCR和多重PCR的方法对60株Aa临床分离菌株的血清型进行鉴定,并分析其与flp-1基因型的关系。结果60株Aa临床分离菌株中血清型c型63.33%,e型23.33%,b型6.67%,a,f型各占3.33%,未检测到d型菌株;在24名被检测者中,15名检测到c型Aa菌株,3名检测到b型菌株,各有2名分别检测到a、e、f型菌株。flp-1基因型Ⅰ型菌株的血清型均为a型,40株Ⅱ型菌株中38株为c型,Ⅳ型菌株均为b型,11株Ⅴ型菌株中9株为e型,Ⅵ型菌株均为e型。结论Aa血清型分布以c型为主,flp-1基因型与菌株血清型存在一定对应关系。Objective To identify the clinically isolated Actinobacillus actinomycetemcofnitans （Aa） serotypes by multiplex PCR, and analyze the relationship between serotypes and tip - 1 classes. Methods Sixty Aa strains were isolated from 24 patients and serotyped by multiplex PCR with primers specific for serotypes a to e, and normal PCR with primers specific for serotype f. The association between serotypes and genotypes of tip - 1 was also analyzed. Results The overall prevalence of serotypes was： c, 63.33%; e, 23.3%; b, 6.67%; and a, f, 3.33%, respectively. Serotype d was not observed among the clinical isolates. Among the 24 patients, 15 patients carried serotype c, 3 patients carried b, 2 patients carried a, 2 patients carried e, and 2 patients carried f. tip - 1 Class Ⅰ was correlated with serotype a. Among 40 strains of tip - 1 Class Ⅱ , 38 strains were serotype c. Strains of tip - 1 Class IV were serotype b. Nine strains were serotype e among 11 strains of tip - 1 ClassⅤ. Serotypes of tip - 1 Class Ⅳ strains were b. Conclusion The most prevalent serotype is e. Serotypes correlate with tip - 1 classes in the majority of strains.

关 键 词：伴放线放线杆菌 血清型 基因 

分 类 号：R781.42[医药卫生—口腔医学]