壳聚糖体外抑菌实验研究  

Inhibition of bacteria by chitosan in vitro

作　　者：刘琨[1] 侯本祥[1] 杨圣辉[1] 李金陆[1] 

机构地区：[1]首都医科大学口腔医学院,北京100050

出　　处：《现代口腔医学杂志》2007年第3期281-283,共3页Journal of Modern Stomatology

基　　金：北京市科技新星计划项目(H013610290113)

摘　　要：目的研究3种壳聚糖(壳聚糖-Zn配合物、壳聚糖-Ag配合物、N,O-羧甲基壳聚糖)对4种感染根管致病菌的抑制作用。方法国际参考菌株选用牙髓卟啉单胞菌、牙龈卟啉单胞菌、中间普氏菌和粘性放线菌,以洗必泰作为对照,采用杯碟法进行抑菌实验,通过观察最小抑菌浓度(MIC)和测量抑菌环直径大小测定实验药物对受试细菌的抑菌作用。结果3种壳聚糖的抑菌效果较强。与对照组洗必泰对各个受试菌的MIC值无统计学差异;1%壳聚糖-Zn配合物、1%壳聚糖-Ag配合物、1%N,O-羧甲基壳聚糖与2%洗必泰的抑菌效果无显著差异。结论3种壳聚糖对根管感染的主要相关菌有显著抑制作用。Objective To test the inhibitory effects on bacteria by three chitosans （chitosan - Zn,chitosan - Ag, N,O- carboxymethyl chitosan）. Methods Porphyromonas endodontalis （P.e）, Porphyromonas gingivalis（P.g）, Prevotella intermedia （P. i）, Actinnomyces viscosus （A. v） were selected for evaluate of the inhibition of bacterium by the Chitosans. Argrose diffusion test was applied and chlorhexidine was served as positive control. The inhibitory effects on the bacteria were evaluated by minimum inhibitory concentration （MIC） and the diameter of inhibitory zone. Results The three chitosans had similar inhibitory effects on bacteria as chlorhexidine had. There was no difference among the three chitosans. Conclusion The three chitosans had strong inhibitory effects on the bacteria usually found infected canals.

关 键 词：壳聚糖 洗必泰 抑菌作用 

分 类 号：R781.05[医药卫生—口腔医学]