超声辐射微泡治疗小鼠皮下H22肝癌移植瘤的疗效  

Therapeutic effects of ultrasound irradiation microbubble agent in thetreatment of subcutaneous H22 hepatoma of mice

作　　者：翟光林[1] 张卫华[1] 姜藻[1] 吴波[2] 吴巍[3] 

机构地区：[1]解放军理工大学通信工程学院门诊部 [2]南京军区南京总医院病理科,江苏南京210002 [3]东南大学公共卫生学院,江苏南京210009

出　　处：《医学研究生学报》2007年第5期477-480,I0003,共5页Journal of Medical Postgraduates

基　　金：国家自然科学基金资助项目(批准号:A040515)

摘　　要：目的:研究超声辐射微泡对小鼠皮下H22肝癌移植瘤的治疗作用。方法:建立小鼠H22肝癌腹水瘤皮下移植瘤模型,随机分对照组、单纯超声治疗组和超声微泡组。观察肿瘤生长情况,计算瘤重,对治疗后肿瘤组织进行病理学检查,了解肿瘤细胞坏死和凋亡情况,并进行定量分析。结果:相对于对照组,超声微泡组肿瘤生长延缓,肿瘤坏死面积显著增加,凋亡肿瘤细胞数明显增多(P<0.05)。结论:超声辐射微泡可显著抑制H22肝癌移植瘤的生长,除杀灭肿瘤细胞外,还可诱导瘤细胞凋亡。Objective ：To investigate the therapeutic effects of ultrasound irradiation microbubble agent in the treatment of subcutaneous H22 ascites cells hepatoma of mice. Methods： H22 ascites cells hepatoma models were established by inoculating tumor cells to subcutaneous tissue of mice. Then, mice were randomly allocated into control group, only ultrasound group, ultrasound and microbubble group. The growth of tumor volume was observed and its weight was calculated. The application of pathological examination was adopted after the treatment. The necrosis area percentage （NAP） was calculated, and the apoptotic index was analyzed with terminal deoxynucleotidyl transferase mediated dUTP nick end lebeling（TUNEL） technique. Results： Compared with the control, the growth of tumor in the ultra-sound and microbubble apoptotic cells number group was slow and the inhibitory effect of tumor was obvious ; Necrosis area and were also increased obviously （ P 〈 0.05 ）. Conclusion ： Ultrasound irradiation microbubble can distinctively inhibit tumor growth. It may not only kill tumor cell but also induce the apoptosis of tumor cells.

关 键 词：超声 微泡剂 肿瘤 

分 类 号：R735.7[医药卫生—肿瘤]