Vif基因体外表达载体构建及其在无细胞系统中的表达  

Cloning and Expression of the HIV Protein Vif in Escherichia coli Cell-free System

作　　者：汪诚[1] 陈海琴[1] 徐志南[1] 殷秀飞 岑沛霖[1] 

机构地区：[1]浙江大学生物工程研究所,杭州310027 [2]浙江养生堂天然药物研究所,杭州310007

出　　处：《科技通报》2007年第3期356-359,367,共5页Bulletin of Science and Technology

基　　金：国家自然科学基金资助项目(No.20276066)

摘　　要：随着-omics(组学)时代的到来,无细胞蛋白质合成系统以具有快速、方便、易于高通量等优点,正在被广泛地研究和应用。本文选取HIV病毒感染因子Vif作为目标蛋白,构建了适宜体外表达的Vif表达载体pIVEX2.4c-Vif,并将其在大肠杆菌无细胞蛋白质合成系统中进行表达,为下一步进行高通量药物筛选奠定了一定的基础。In the new era of ＂-omics＂, the cell-free expression system opens a new strategy in protein expression with advantages of rapid, convenient and high-throughput expression. Cell-free synthesis system enables the direct in vitro expression of protein from template DNA or mRNA. We choose Vif as a target protein, an expression vector pIVEX2.4c-Vif was constructed for the cell-free expression of Vif（virion infectivity factor）, and the target protein was synthesized in the batch-mode E. coli cell-free system. This result is the cornerstone of the pharmaceutical high throuhgput process.

关 键 词：HIV 生物合成 无细胞合成系统 Vif(virion infectivity factor) 

分 类 号：Q78[生物学—分子生物学]