生长抑素对人胃癌细胞BGC-823生长的调控作用  

Effects and signal transduction of Somatostatin on human gastric cancer cell line BGC-823

作　　者：徐秀英[1] 张岱[1] 姜若兰[1] 卢香兰[2] 宗志宏[3] 

机构地区：[1]中国医科大学第一临床学院消化科,辽宁沈阳110004 [2]中国医科大学第一临床学院血液室,辽宁沈阳110004 [3]中国医科大学基础医学院生化教研室,辽宁沈阳110004

出　　处：《中国现代医学杂志》2007年第9期1037-1040,1045,共5页China Journal of Modern Medicine

摘　　要：目的观察生长抑素对人体分化胃癌细胞株BGC-823的生长调控作用,测定细胞内环磷酸腺苷(cAMP)含量及蛋白激酶C(PKC)活性,探讨受体后信息传导途径。方法BGC-823细胞在含10%小牛血清的RPMI 1640培养液中,于37℃、5%CO2条件下培养,分别加入不同浓度的生长抑素,应用MTT法观察细胞的增殖程度。应用放射免疫分析方法测定细胞内cAMP含量,应用[γ-32p]ATP掺入外源性底物的方法测定PKC活性。结果生长抑素能抑制BGC-823细胞的生长,且与剂量呈正相关。生长抑素能抑制细胞PKC活性,而对细胞内cAMP生成无明显影响。结论1、生长抑素能抑制胃癌细胞BGC-823的生长。2、生长抑素可降低PKC活性,提示其受体后信息传递途径可能涉及DG-PKC系统。[Objective] To observe the effects of Somatostatin on human poorly differentiated cell line BGC-823 and to determine the intracellular cyclic adlnosine monophosphate （cAMP） concentration and the activity of proteinkinase C（PKC）. [Methods] BGC-823 cells were cultured in RPM1 1640 medium containing 10% FCS in atmosphere of 5% carbon dioxide at 37℃, and then were treated with Somatostatin in different cencentrations. The viability and proliferation were determined with Mosmann＇s method（MTT）. The intracellular cAMP concentrations were determined by radioimmunoassay （RIA）; the activity of PKC was determined by incorporation [γ-^32p]-ATP into exogenous substrate. [Results] Somatostatin can inhibit the growth of BGC-823 cell in dose-dependent fashion. Somatostatin can increase the intracellular PKC activity in a dose-dependent manner, but can not affect the intracellular cAMP level. [Conclusion] Somatostatin can inhibite the growth of human poorly differentiated gastric carcinoma cell line, BGC-823, and DG-PKC may involve in the signal transduction.

关 键 词：生长抑素 环磷酸腺苷 蛋白激酶C 胃癌 调控作用 

分 类 号：R735.2[医药卫生—肿瘤]