结核杆菌抗原Ag85B诱导抗黑素瘤免疫应答及其机制研究  

Induction and mechanism study of anti-melanoma immune response by M.tuberculosis Ag85B

作　　者：吴学忠[1] 魏海明[2] 郑晓东[2] 金腾川[2] 田志刚[2] 

机构地区：[1]山东省皮肤病性病防治研究所,山东济南250022 [2]中国科学技术大学生命科学学院免疫学研究所,安徽合肥230027

出　　处：《细胞与分子免疫学杂志》2007年第1期36-38,共3页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金杰出青年基金项目(30125038);国家重点基础研究发展规划(973)资助项目(2001CB510009)

摘　　要：目的:探讨Ag85B在诱发抗黑素瘤免疫应答中的作用,以及其用于肿瘤免疫治疗的可行性。方法:经背部皮下接种黑色素瘤细胞株B16建立C57BL/6小鼠荷瘤模型,并分为对照1、2组和实验组。于荷瘤模型建立后,对照1、2组经背部皮下分别接种B16细胞和B16/pcDNA3细胞,实验组背部皮下接种B16/pcDNA3-Ag85B细胞。分别观测3组小鼠肿瘤的体积、生存时间和血清中的IFN-γ及IL-4的含量。研究Ag85B在诱导抗肿瘤免疫应答中的作用。结果:从13d到23d,对照1组和对照2组荷瘤小鼠,肿瘤的平均体积分别从1.1058cm3和0.9123cm3增长到7.5983cm3和5.8746cm3;而实验组荷瘤小鼠,肿瘤的平均体积由0.5158cm3增长到1.5080cm3。对照1组和对照2组荷瘤小鼠的平均生存时间分别为24.1d和24.7d,而实验组荷瘤小鼠的平均生存时间为27.8d。13d内,两对照组和实验组小鼠血清IFN-γ的含量均有所上升(对照1组、对照2组和实验组分别上升至26.3ng/L、23.0ng/L和25.2ng/L),随后两对照组小鼠血清IFN-γ的含量均呈下降趋势(对照1组和对照2组分别下降至19.3ng/L和18.3ng/L),而实验组小鼠血清IFN-γ的含量则呈上升趋势(上升至46.5ng/L)。13d内,两对照组和实验组小鼠血清IL-4的含量均有轻微升高,随后逐渐下降,但3组小鼠血清IL-4含量的变化差异不明显。结论:接种B16/pcDNA3-Ag85B细胞的实验组小鼠血清中IFN-γ的含量明显增高,并可抑制荷瘤小鼠体内肿瘤的生长,延长荷瘤小鼠生存的时间。AIM： To study the anti-melanoma immunity efficacy of Ag85B antigen gene therapy in vivo. METHODS： C57BL/6 mice were inoculated s. c. with B16 cells, and 8 days later the mice were inoculated s. c. again with B16 cells （control group 1）, B16/pcDNA3 cells （control group 2） or B16/pcDNA3-Ag85B cells （experimental group）, respectively. Tumor volume, survival time, serum IFN-γ level and IL-4 level of 3 groups mice were observed. Antitumor activity of Ag85B was studied. RESULTS： From 12 to 23 day, the mean tumor volume of mice increased from 1.1058 cm^3 and 0. 9123 cm^3 to 7.5983 cm^3 and 5.8746 cm^3 in the control group 1 and 2, respectively. But it increased from 0. 5158 cm^3 to 1.5080 cm^3 in the experimental group. The mean survival time of mice was 24. 1 days and 24. 7 days in the control group 1 and 2, respectively. That was 27. 8 days in the experimental group. Within 13 days after the last inoculation, the serum IFN-γ level of all groups experienced increased （That increased to 26. 3 ng/L, 23.0 ng/L and 25.2 ng/L in the control group 1,2 and the experimental group, respectively）. Subsequently, the serum IFN-γ level in the two control groups decreased （That decreased to 19. 3 ng/L and 18. 3 ng/L in the control group 1 and 2） while it still augmented in the experimental group（That increased to 46.5 ng/L）. IL-4 level was slightly but not significantly enhanced and then declined in all mice. CONCLUSION： Ag85B induced the increase of serum IFN-γ level in the animals experiments, inhibited the tumor growth and prolonged the survival of the tumor-bearing mice.

关 键 词：pcDNA3-Ag85B B16细胞 C57BL/6 肿瘤免疫 

分 类 号：R730.51[医药卫生—肿瘤]