吗啡对小鼠海马结构突触素表达及突触结构的影响  

Influence of morphine on synaptophysin expression and synapse structure in hippocampus of mice

作　　者：陈良富[1] 柳洁[1] 李莉[1] 苏筱玲[1] 

机构地区：[1]怀化医学高等专科学校组织学与胚胎学教研室,怀化418000

出　　处：《第二军医大学学报》2007年第5期484-487,共4页Academic Journal of Second Military Medical University

基　　金：湖南省教育厅科学基金(04C051).~~

摘　　要：目的:研究吗啡对海马结构突触素(SYN)表达的影响,为吗啡慢性中毒、成瘾和戒断症状的发生与治疗提供病理学依据。方法:将20只小鼠随机分成对照组和实验组,每组10只。对照组每只小鼠每日皮下注射生理盐水0.1 ml,实验组每只小鼠每日皮下注射吗啡0.1 ml(1 mg)。注射30 d后,将两组小鼠断头处死,开颅取出脑组织并制片。切片经免疫组织化学SP法处理,光镜观察与照相,图像分析系统分析,最后行统计学处理。结果:在对照组,内嗅区、下托、齿状回的分子层和多形层及海马的始层和辐射层均显黄色,有SYN阳性表达,海马的腔隙分子层显淡黄色,有SYN弱阳性表达,锥体细胞和颗粒细胞的胞膜显黄色,呈SYN阳性表达,海马结构的平均灰度值为132.84±8.67;下托和内嗅区内有阳性表达神经元,其均值为(7.80±1.03)/mm^2。在实验组,下托和齿状回多形层显黄色,有SYN阳性表达,内嗅区及海马多形层、辐射层和腔隙分子层显深黄色,有SYN强阳性表达,锥体细胞和颗粒细胞的细胞膜显深黄色,呈SYN强阳性表达,海马结构的平均灰度值为116.27±5.70;下托和内嗅区内有强阳性表达神经元,其均值为(11.90±1.45)/mm^2。实验组的SYN阳性神经元比对照组多,SYN的表达比对照组强(P<0.01)。海马结构呈毛玻璃状,细胞染色浅,其中可见较多固缩的细胞核与空泡。结论:吗啡能促进海马结构内神经元的SYN表达和凋亡,影响突触的结构和功能。Objective： To study the influence of morphine on the expression of synaptophysin （SYN） and synapse structure in mice hippocampus,so as to provide pathological evidence for studying the development and treatment of chronic morphine intoxication, addiction and abstinence symptoms of morphine. Methods： Twenty mice were evenly randomized into control group and experiment group. Mice in control group were injected with normal saline （0. 1 ml daily） and those in experimental group were injected with morphine （0.1 ml, 1 mg daily）. Thirty days later the mice in 2 groups were killed and their brain tissues were harvested and made into slices,stained with immunohistochemical techniques （SP） and photographed under the light microscope. The images were analyzed with the image analytical system and the data were statistically analyzed. Results： In the control group,positive staining of SYN was found in the entorhinal area, subiculum, stratum plextiforme, polymorphic layer of gyrus dentatus, stratum oriens, and stratum radiatum of hippocampus; weak positive staining of SYN was noticed in the stratum lacunosum-moleculare of hippocampus; positive staining of SYN was also found the membrane of pyramidal cells and granule cells, with the mean gray scale value of the hippocampal structure being 132.84 ± 8.67. Positively stained neurons was also found in the entorhinal area and the subiculum, with a intensity of （7.80± 1.03）/ mm^2. In the experiment group,the subiculum and polymorphic layers of gyrus dentatus were positively stained for SYN; the entorhinal area, stratum oriens, stratum radiatum and stratum lacunosum moleculare of hippocampus were strongly positive of SYN; the membrane of pyramidal cells and granule cells were also strongly positive of SYN, with the mean gray scale value of the hippocampal structure being 116.27 ±5.70. Strongly stained neurons were also found in the entorhinal area and the subiculum, with the intensity being （11.90± 1.45）/mm^2. The number of SYN positive neurons and

关 键 词：吗啡 突触素 突触 海马 

分 类 号：R749.61[医药卫生—神经病学与精神病学]