贻贝凝集素抗-HIV活性研究  

Anti-Human Immunodeficiency Virus Type 1(HIV-1 IIIB) Activity of Lectin from Sea Mussel Crenomytilus Grayanus

作　　者：李伟[1] 熊川男[2] 王建华[3] 郑永唐[3] 金桥[1] 佟长青[1] 曲敏[1] 刘洪伟[1] 汪秋宽[1] 

机构地区：[1]辽宁省水产品加工与贮藏重点开放试验室大连水产学院食品工程系,辽宁大连116023 [2]中国科学院大连化学物理研究所生物技术部 [3]中国科学院昆明动物研究所分子免疫药理学试验室,昆明650223

出　　处：《沈阳农业大学学报》2007年第2期207-210,共4页Journal of Shenyang Agricultural University

基　　金：辽宁省自然科学基金项目(2050885);辽宁省教育厅资助项目(05L086);农业部暨辽宁省省级高校重点开放试验室开放课题(K2006-09);大连水产学院科研项目(015690)

摘　　要：分离提取海洋无脊椎动物贻贝(Crenomytilus grayanus)凝集素,考察其抗HIV活性。采用半乳糖-Sepharose 6B亲和层析和Sephacryl S-200层析分离提取贻贝凝集素(Crenomytilus grayanus lectin,CGL),以光镜检查合胞体抑制试验,以ELSA测定HIVp24抗原表达水平。从海洋无脊椎动物贻贝中分离出的凝集素(CGL),为N-乙酰半乳糖胺/半乳糖(GalNAc/Gal)特异性的凝集素。CGL在27.88mg.L-1浓度时,对HIV诱导细胞病变的抑制达50%;在45.70mg.L-1时,对HIV-1复制的抑制达50%;同时在35.12mg.L-1浓度时,对HIV感染细胞融合的阻断达50%。CGL, a Crenomytilus grayanus lectin was purified from sea mussel C. grayanus, and the anti-HIV activity was assessed. CGL was purified with affinity chromatograph on galactose-Sepharose 6B and following gel filtration on Sephacryl S-200. The inhibition of syncytial cell formation induce by human immunodeficiency virus was determined under microscope, and the reduction of HIV p24 antigen expression level was measured with ELISA. The purified CGL showed GalNAc/Gal specificity. CGL inhibited cytopathic effect induced by HIV-1 and the production of viral p24 antigen. The EC50 values were 27.88 and 47.5 mg·L^-1, respectively. CGL could block the cell-to-cell fusion process of HIV infected and uninfected cells with ECso values of 35.12 mg·L^-1. CGL is a GalNAc/Gal specific lectin with anti-HIV activity.

关 键 词：凝集素 抗-HIV活性 贻贝 N-乙酰半乳糖胺 半乳糖 

分 类 号：Q946.1[生物学—植物学]