维生素E、亚硒酸钠对非酒精性脂肪肝作用的实验研究  

Experimental study about the effect of Vitamin E and sodium selenite on nonalcoholic fatty liver

作　　者：谭丽[1] 管小琴[1] 王倩[1] 

机构地区：[1]重庆医科大学基础医学院病理教研室,重庆400016

出　　处：《重庆医科大学学报》2007年第6期606-609,614,共5页Journal of Chongqing Medical University

摘　　要：目的:探讨维生素E(VitE)、亚硒酸钠抗非酒精性脂肪肝(Nonalcoholic fatty liver,NAFL)的作用机理。方法:24只大鼠随机分成三组:模型组、药物组、对照组,分别喂饲高脂饲料、干预饲料、普通饲料。全部动物于5周末处死。观察病理形态,血清及组织匀浆测超氧化物歧化酶(SOD)和丙二醛(MDA),免疫组化观察组织中核因子kB(NF-kB)、肿瘤坏死因子α(TNF-α)蛋白的表达;结果:①与对照组比较,模型组血清及组织中SOD降低,MDA增高;肝脏NF-kB、TNF-α蛋白表达水平显著上升。②与模型组比较,药物组血清及组织中SOD增高,MDA降低;肝脏NF-kB蛋白表达水平下降,TNF-α无显著变化。结论:联合适量补充VitE+亚硒酸钠能增强SOD活力,降低NF-kB蛋白的表达,这可能是其有效防止NAFL发生的重要作用机制之一。Objective：To study the effect of Vitamin E （VitE）and Sodium selenite on nonalcoholic fatty liver （NAFL）.Methods： 24 SD rats were randomly divided into 3 groups. The model group,treatment group and contrast group were respectively fed with high fat diet,interfering diet and normal diet.All animals were sacrificed at the end of the 5th week, The liver pathology was observed under the light microscope. Superoxide dismutase （SOD）,malondialdehyde （MDA）were determined by biochemistry analysis.The expressions of nuclear factor kappa B（NF-kB）and tumor necrosis factor alpha（TNF-α ）proteins in hepatocytes were examined by immunohistochemistry, Resu/t： ①compared with the contrast group,serum and liver SOD levels decreased in modal group,while MDA were raised.The expressions of NF-kB and TNF-α proteins in liver tissue increased significantly in model group. ②compared with the model group,serum and liver SOD level increased in treatment group,while MDA was lowered. The expression of NF-kB proteins in liver tissue was reduced in treatment group,and no significant changes occured in TNF-α protein expression. Conclusions：Combination of sufficient quantum of VitE and Sodium selenite can improve the SOD activities and reduce the expression of NF-kB proteins in liver tissue,which is possibly the important mechanism for VitE and Sodium selenite to prevent NAFL.

关 键 词：非酒精性脂肪肝 核因子KB 肿瘤坏死因子Α 维生素E 硒 

分 类 号：R361.2[医药卫生—病理学]