维生素对谷氨酸棒杆菌SYPS-062直接发酵合成L-丝氨酸的影响  

Effects of Vitamins on the Direct Fermentative Production of L-serine in Corynebacterium glutamicum SYPS-062

作　　者：张晓娟[1] 窦文芳[2] 许泓瑜[2] 许正宏[1] 

机构地区：[1]江南大学工业生物技术教育部重点实验室,无锡214036 [2]江南大学生物工程学院,无锡214036

出　　处：《中国生物工程杂志》2007年第5期50-55,共6页China Biotechnology

基　　金：国家"973"计划资助项目(2007CB707804);长江学者和创新团队发展计划资助项目(IRT0532)

摘　　要：研究了VB1,生物素,VB6,VB2,叶酸和VB12对一株谷氨酸棒杆菌(Corynebacterium glutamicum)SYPS-062直接利用糖质原料发酵生产L-丝氨酸的影响,并且初步分析了这几种维生素对菌株SYPS-062发酵积累L-丝氨酸的调控机制。添加一定量的生物素,VB1和VB6表现出对L-丝氨酸积累分别为35%,28%和11%的促进;添加VB2实现了L-丝氨酸和生物量的等幅提高;而叶酸和VB12则通过促进菌株SYPS-062中1C单元循环的效率使L-丝氨酸的积累量分别提高了39%和82%,并且实现了产物转化率(YP/S)及单位细胞产率(YP/X)的显著提高。将6种维生素在其分别的最优浓度下复配,添加在发酵培养基中,结果发现发酵周期有6h左右的缩短,并且达到的最大生物量及L-丝氨酸的积累分别为11g/L和9.0g/L。The effects of six vitamins on a novel isolate, Corynebacterium glutaraicum SYPS-062, which could directly produce L-serine from carbohydrates were investigated. The results show that all these tested vitamins- VB1, biotin, VB2, VB6, folate and VB12 could enhance the growth of C. glutamicum SYPS-062 and improve the production of L-serine. However, the mechanisms of their metabolic regulations were different. Although VB1, biotin and VB6 could enhance the L-serine degradation, the significant increasing of biomass induced the increment of L-serine accumulation, which was 31% ,28% and 11% higher than the control; VB2 improved both the biomass and the L-serine production equally, however didn＇t influence the distribution of metabolic pathway of L-serine; folate and VB12 could diminish L-serine degradation flux ratio and increase the YP/S and YP/X by accelerating the efficiency of the one carbon units metabolism. The final L-serine productions were 39% and 82% higher than the control. Finally, the fermentation period was 6 h shorter by adding all these vitamins at their optimal doses than by adding biotin only, and the accumulation of L-serine and the biomass were 9.0g/L and 11g/L, which were 70% and 76% higher than the control.

关 键 词：L-丝氨酸 谷氨酸棒杆菌 维生素 代谢调 控优化 

分 类 号：TQ922.9[轻工技术与工程—发酵工程]