转基因草莓向栽培草莓中转移CBF1基因的研究  

Study on CBF1 Gene Transferred from Transgenic Strawberry to Nontransgenic Strawberry

作　　者：刘燕[1] 胡鸢蕾[2] 董静[1] 田自华[3] 陈梅香[1] 金万梅[1] 

机构地区：[1]北京市农林科学院林业果树研究所,北京100093 [2]北京大学生命科学学院,北京1000871 [3]内蒙古农业大学农学院,呼和浩特010019

出　　处：《分子植物育种》2007年第3期309-313,共5页Molecular Plant Breeding

基　　金：北京市自然科学基金项目(5052010)资助。

摘　　要：为了提高草莓“丰香”的抗寒能力,我们以转CBF1基因的草莓“哈尼”为父本,以普通栽培品种“丰香”为母本进行杂交,获得杂交后代。种子经抗生素筛选后,阳性株系通过PCR检测,在杂交后代中可扩增出一条640bp的目的基因条带,说明通过有性杂交可以将转基因草莓中的外源基因CBF1转移到普通的栽培品种当中。采用电解质渗漏的方法对转育成功的植株进行了抗寒性鉴定,结果表明:转育成功植株的电导率低于对照植株的电导率,在-20℃时转育成功植株的电导率有一个跃升,而未经过转育的植株在-16℃时的电导率就明显增加;同时研究了对照与转育材料的细胞胞间结冰温度,发现转育成功植株的胞间结冰温度比对照植株的低约2℃,表明通过有性杂交转育CBF1基因的植株抗寒性提高了。In order to improve strawberry Toyonoka freezing resistance, transgenic strawberry Honeoye which was studied as father and cultivar Toyonoka which was studied as mother was crossed. Results of molecular analysis by PCR confirmed that foreign gene CBF1 in transgenic strawberry Honeoye could be transferred into cultivar Toyonoka by crossing. Electrolyte leakage tests showed that electrolyte leakage ratio of transferred CBF1 gene plants was lower than control s at every low temperature. Comparaed with cultivar Toyonoka whose electrolyte leakage ratio rised obviously at -16℃, electrolyte leakage ratio of transferred CBF1 gene plants rised obviously at -20℃, and the intercellular icing temperature of transferred CBF1 gene plants is 2℃ lower than control s. The study showed that the expression of CBF1 gene of crossed plant increased the freezing tolerance of transgenic strawberry.

关 键 词：草莓 CBF1基因 转育 抗寒性 

分 类 号：S668.4[农业科学—果树学]