C-erbB-2蛋白和缝隙连接蛋白43在胃癌组织中的表达及意义  

Expression of C-erbB-2 and Cx43 protein in gastric carcinoma

作　　者：李磊[1] 刘俊[1] 钱伟[1] 王宏志[1] 宋慧[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院内消化内科,湖北武汉430022

出　　处：《胃肠病学和肝病学杂志》2007年第2期132-135,共4页Chinese Journal of Gastroenterology and Hepatology

摘　　要：目的探讨C-erbB-2癌蛋白和缝隙连接蛋白43(connexin,Cx43)在腺型胃癌中的表达并分析其临床意义。方法应用免疫组织化学SP法分别检测C-erbB-2癌蛋白、Cx43在48例腺型胃癌和26例胃炎组织中的表达,并分析其与临床病理参数的关系。结果C-erbB-2癌蛋白、Cx43阳性呈棕黄色颗粒定位于细胞膜或细胞质;C-erbB-2癌蛋白在胃癌中的阳性表达率(50.0%)显著高于胃炎黏膜组织的阳性率(2χ=6.70,P<0.05);Cx43在胃癌中阳性率(43.8%)显著低于胃炎黏膜组织的阳性率(2χ=23.03,P<0.05);C-erbB-2癌蛋白的表达与胃癌的大小、分化程度及淋巴结转移呈显著相关(2χ=6.00、9.41、5.49,P值均<0.05);Cx43蛋白表达降低或缺失同样与胃癌的分化程度及淋巴结转移呈显著相关(2χ=7.49、6.86,P值均<0.05);C-erbB-2癌蛋白和Cx43蛋白呈负相关(2χ=0.01,r=-0.378,P<0.05)。结论C-erbB-2癌蛋白的高表达、Cx43表达降低或缺失与胃癌分化程度、浸润转移密切相关,联合检测C-erbB-2和Cx43有助于判断胃癌的生物学行为。Objective To explore the expression of C-erbB-2 and Cx43 protein in gastric carcinoma. Methods The expression of C-erbB-2 and Cx43 protein in 48 cases with gastric carcinoma and 26 cases with gastritis were examined by S-P immunohistochemical method,and the relationship between their expression and clinical-pathological parameters was analyzed. Results C-erbB-2 and Cx43 proteins were detected on cell membrane and in cytoplasm. The total positive rates of C-erbB-2and Cx43 protein staining in 48 cases of gastric carcinoma were 50.0% （24/48） and 43.8% （21/48） respectively. The high level of C-erbB-2 expression and the low level of Cx43 expression were positively correlated with tumor size, differentiation degree and lymph node metastasis in gastric carcinoma （ P 〈 0.05 ） o There was a negative relationship between the expression of C-erbB-2 and Cx43 （ r = - 0. 378,P 〈 0.05）. Conclusion The positive expression of C-erbB-2 and negative expression of Cx43 were correlated with tumor differentiation degree and lymph node metastasis. The combined detection of C-erbB-2 and Cx43 protein may be valuable in predicting the biological behavior of gastric carcinoma.

关 键 词：胃癌 C-ERBB-2 缝隙连接蛋白43 免疫组织化学 

分 类 号：R735.2[医药卫生—肿瘤]