磷酸化AKT、AKT2、PTEN在NSCLC中的表达及意义  

The Expression and Clinical Significance of phospho-AKT,AKT2 and PTEN Proteins in Human Non-small Cell Lung Cancer

作　　者：苗丽君[1] 孙振涛[2] 王静[1] 吴秋歌[1] 吴逸明[3] 吴拥军[3] 

机构地区：[1]郑州大学第一附属医院呼吸内科,450052 [2]郑州大学第一附属医院麻醉科,450052 [3]郑州大学公共卫生学院

出　　处：《肿瘤防治研究》2007年第5期328-330,共3页Cancer Research on Prevention and Treatment

摘　　要：目的探讨AKT信号转导通路中有关蛋白磷酸化AKT(p-AKT)、AKT2及PTEN在非小细胞肺癌(NSCLC)中的表达、相关性及意义。方法应用免疫组化的方法,检测80例NSCLC组织及35例非肿瘤性肺组织标本中p-AKT、AKT2、PTEN的表达,并与临床病理因素进行相关性分析。结果p-AKT、AKT2在NSCLC中高表达,阳性率分别为78.8%、91.3%,显著高于非肿瘤性肺组织中的0、5.7%(P<0.05)。PTEN在NSCLC中低表达,阳性率47.5%,显著低于非肿瘤性肺组织中的94.3%(P<0.05)。p-AKT的表达与患者年龄、性别、组织学类型及分化程度、TNM分期、淋巴结转移无关(P>0.05)。AKT2的表达与淋巴结转移有关,在淋巴结转移组的阳性表达率100%显著高于非淋巴结转移组表达率80.6%(P<0.05)。PTEN表达与淋巴结转移、组织的分化程度有关(P<0.05)。AKT2与p-AKT呈正相关(rs=0.596,P<0.01)。PTEN表达与p-AKT(rs=-0.240,P<0.05)、AKT2(rs=-0.326,P<0.01)呈负相关。结论在NSCLC中存在AKT的活化,PTEN表达的丢失与AKT的活化有关,AKT2是PTEN的调节形式之一。Objective To investigate the expression, clinical significance and relationship of phospho AKT （p-AKT）, AKT2 and PTEN in human non-small cell lung cancer （NSCLC） tissue. Methods The expression of p-AKT ,AKT2 and PTEN in 81） cases of NSCLC and 35 cases of no-cancerous lung disease were assessed by immunohistochemistry, and their correlations with clinicopathologic factors were statis tieally analyzed. Results The positive rate of p-AKT, AKT2 was 78.8%.91.3% in NSCI.C and 0%. 5. 7% （P〈0. 05） in no-cancerous lung disease. But the positive rate of PTEN （47. 5 %） was significantly lower than that of PTEN （94. 3%） in no-cancerous lung disease （P%0. 05）. The expression of p-ANT didn＇t relate to age, sex, histological subtype and tumor differentiation, lymph node metastasis and TNM stages（P〉0. 05）. The positive rate of AKT2 in the group with lymph node metastasis （100. 0 % ） was significantly higher than that of AKT2 in the group without lymph node metastasis （80. 6% ） （P〈 0. 05）. The expression of PTEN correlated with lymph node metastasis and differentiation of NSCLC （P〈0. 05）. In addition, Positive correlations were observed between the expression of ANT2 and p-ANT in NSCLC tissues（P〈0. 05）. The expression of PTEN had negative correlations with the expression of p-AKT and AKT2 （P〈0. 05）. Conclusion AKT activation may be present in NSCLC. The loss of expression of PTEN may correlate to activation of ANT. ANT2 may be one of regulated forms of PTEN.

关 键 词：非小细胞肺癌 P-AKT AKT2 PTEN 

分 类 号：R734.2[医药卫生—肿瘤]