G-CSF动员循环间充质干细胞及其对小鼠颅脑损伤修复的可行性分析  

Feasibility of MSCs mobilization by G-CSF and its prosthetic effect in traumatic brain injury

作　　者：邓均[1] 艾国平[1] 周桃莉[2] 王军平[1] 徐辉[1] 邹仲敏[1] 董世武[1] 郝磊[1] 冉新泽[1] 粟永萍[1] 

机构地区：[1]第三军医大学军事预防医学院防原医学教研室全军复合伤研究所创伤,烧伤与复合伤国家重点实验室,重庆400038 [2]第三军医大学基础医学部病原生物学教研室,重庆400038

出　　处：《第三军医大学学报》2007年第12期1131-1134,共4页Journal of Third Military Medical University

基　　金：国家重点基础研究发展规划项目("973"项目)(2005CB522605);国家自然科学基金(30200142)~~

摘　　要：目的建立小鼠严重颅脑创伤模型探讨粒细胞集落刺激因子(granulocyte colony-stimulating factor,G-CSF)动员循环间充质干细胞(mesenchym alstem cells,MSCs)的可行性,并观察G-CSF动员的修复效应。方法以G-CSF作为动员剂,培养骨髓和外周血来源的MSCs,计数动员前后的CFU-F。制作小鼠严重颅脑创伤模型,致伤后采用G-CSF对MSCs进行动员,在2、24、48、96、120、144、192、264、336h进行神经行为学评分、运动功能评分,并观察小鼠死亡率和病理组织切片。结果从小鼠外周血培养出MSCs,G-CSF动员后骨髓和外周血MSCs的CFU-F数量显著高于正常对照组(P<0·01)。致伤后小鼠神经行为学和运动功能评分显著低于正常对照组(P<0·01),创伤治疗组在144～192h期间评分显著高于创伤组(P<0·05)。致伤后小鼠死亡率增高,病理切片可见创伤处有出血和空泡。结论成功培养及用G-CSF动员了外周血MSCs,在成功建立小鼠严重颅脑创伤模型的基础上,显示G-CSF动员可以降低严重颅脑创伤小鼠死亡,参与了创伤修复。Objective To explore the feasibility of mobilization circulating MSCs by G-CSF and observe the repairing effect of G-CSF mobilization in severe mouse traumatic brain injury （TBI） model. Methods MSCs-derived bone marrow and peripheral blood （PB） were cultured and its CFU-F were counted after mobilization by G-CSF. At 2, 24, 48, 96, 120, 144, 192, 264, 336 h after severe TBI in mice was establish, the neurobehavior of mice was measured by neurological examination and motor functional test, and mortality rate and pathologic changes were analyzed. Results MSCs-derived PB were successfully cultured. The CFU-F of mobilization group increased significantly than that of control group （P 〈 0. 01 ）. After TBI, the neurobehavioral and motor function scores decreased significantly as compared with that of normal group （ P 〈 0. 01 ） , the scores of treatment group increased significantly as compared with that of trauma group （ P 〈 0. 05 ）. The mortality rate was high after TBI, and brain injury such as haemorrhage and vacuolus were confirmed by pathologic examinations. Conclusion G-CSF can mobilize MSCs-derived PB. G-CSF mobilization can decrease the death of TBI mice and participate repair in trauma of TBI mice.

关 键 词：间充质干细胞 创伤 动员 G-CSF 

分 类 号：R329.21[医药卫生—人体解剖和组织胚胎学] R331.22[医药卫生—基础医学]