不同剂量γ射线照射诱发CHL细胞基因组不稳定性的研究  

STUDY OF GENOMIC INSTABILITY OF CHL CELLS INDUCED BY DIFFERENT DOSE OF γ RAY

作　　者：左雅慧[1] 郭鲜花[1] 王小莉[1] 王仲文[1] 耿晓华[2] 

机构地区：[1]中国辐射防护研究院,太原030006 [2]山西大学,太原030006

出　　处：《辐射防护》2007年第3期145-150,共6页Radiation Protection

基　　金：中核基金资助项目(项目号:416050202)

摘　　要：采用单细胞凝胶电泳技术和微核检测技术对60Coγ射线照射诱发中国仓鼠肺细胞(CHL细胞)产生的直接效应、基因组不稳定性进行了初步探讨。将对数生长期的细胞分成不同的剂量组,经γ射线照射后,对受照存活细胞进行单细胞凝胶电泳(SCGE)和微核发生率(MF)的测定。同时,将一部分受照存活细胞继续传代培养,待其传至33代时,再给予2 Gy的照射剂量进行二次照射,然后再进行单细胞凝胶电泳和MF的测定。结果表明,首次照射剂量与子代二次照射后的损伤程度存在剂量-效应关系。本实验为探讨辐射诱发基因组不稳定性的研究提供了实验基础。In this paper, the direct effect of lung fibroblasts（CHL） cell in China hamster and genomic instability induced by ^60Co γ rays was studied by the methods of single cell gel electrophoresis（SCGE） and Cytokinesis-block micronucleus assay . The fast-growing cells were grouped and then irradiated by different doses of gamma ray. Parts of surviving cells were studied by the methods of single cell gel electrophoresis （SCGE） and micronucleus frequency （MF）. At the same time, the others were continuously cultured. After 33 passages of subculture, the progenies were secondly irradiated with 2 Gy gamma rays. And then SCGE and MF were performed. The results showed that the second damage was correlated with the original irradiation dose. It could be concluded that the gamma rays not only induce the immediate biological effects in CHL cells, but also can lead to the genomic instability in the descendants.

关 键 词：基因组不稳定性 ^60CO Γ射线 二次损伤 微核 单细胞凝胶电泳 

分 类 号：R144[医药卫生—公共卫生与预防医学]