脑胶质瘤中P33^(ING1)和PINCH蛋白的表达及意义  

Expression and significance of P33^(ING1) and PINCH in brain gliomas

作　　者：焦保华[1] 代彬[1] 姚志刚[2] 

机构地区：[1]河北医科大学第二医院神经外科,石家庄市050000 [2]石家庄市第三医院神经外科

出　　处：《河北医药》2007年第1期16-18,共3页Hebei Medical Journal

摘　　要：目的检测P33ING1及PINCH蛋白在脑胶质瘤中的表达,探讨其在胶质瘤发生、发展中的作用。方法选取行脑胶质瘤手术切除的新鲜胶质瘤标本50例,应用S-P免疫组织化学方法检测P33ING1和PINCH在瘤组织及正常组织中的表达。结果50例胶质瘤标本中P33ING1阳性表达率为90%(45/50),正常组织中全部表达阳性(100%);随着肿瘤级别的增高,P33ING1表达水平下降,2者呈负相关(P<0.01)。PINCH蛋白的阳性表达率为76%(38/50),正常组织中有3例表达,阳性率为37.5%(3/8);随着肿瘤级别的增高,PINCH表达水平越高,2者呈正相关(P<0.01)。P33ING1与PINCH在胶质瘤中的蛋白表达水平(LI)呈明显的负相关(r=-0.5422,P<0.01)。结论抑癌基因P33ING1的表达对脑胶质瘤细胞增殖起抑制作用,而作为信号连接蛋白PINCH的表达则对胶质瘤的侵袭与转移起促进作用。P33ING1与PINCH的表达存在相关性,是影响脑胶质瘤发生发展的重要因素。Objective To detecte expression of P33^ING1 and PINCH in brain gliomas and investigate their relationship with occurance and invasive growth of gliomas. Methods 50 fresh samples of gliomas were collected, and stains were used to examine the expressionof P33^ING1 and PINCH. The SPSS 11.5 was used for statistical analysis. Results 45 of 50 （90%） cases were positive in gliomas, and 100% in normaltissue. The difference in P33^ING1 was significantly different in every gradeand their expression levels were decreased with increasing grade progress of gliomas. The percentage of PINCH positive cells expression was 76% in gliomas and 37.5% in normal tissue. The expression of PINCH was significantly increased with increasing grade progress of gliomas. Labelling index （LI） of P33^ING1 expressionwas negatively correlated with PINCH expression. Conclusions Suppressive gene of cancer, P33^ING1, has inhibitory effect on proliferation ofgliomas. But as a signal - linked protein, the expression of PINCH has accelerativeeffect on cell invasion and metastasis. The positive adjustment of PINCH and the negative adjustment of P33^ING1 are important factors to affect the occurance and development of gliomas.

关 键 词：胶质瘤 抑癌基因 侵袭与转移 P33^ING1 PINCH 

分 类 号：R739.91[医药卫生—肿瘤]