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作 者:李明波[1] 胡生发[2] 黄良[1] 黄菊芳[1] 罗学港[1]
机构地区:[1]中南大学湘雅医学院人体解剖与神经生物学系,湖南长沙410013 [2]中南大学湘雅医院眼科激光中心,湖南长沙410008
出 处:《现代生物医学进展》2007年第6期827-829,共3页Progress in Modern Biomedicine
基 金:国家自然科学基金(30100098;30570979)
摘 要:目的:检测急性眼高压后大鼠视网膜GLT-1的表达变化。方法:成年大鼠左眼眼压升高至闪光视网膜电图b波消失的临界眼压且维持缺血60min。实验动物分别存活1、3、7或14d后通过免疫组织化学检测大鼠视网膜GLT-1的表达变化,尼氏染色检测节细胞的变化。结果:急性眼高压后视网膜随着再灌时间的延长,内层视网膜厚度逐渐变薄,节细胞层细胞数目进行性下降。GLT-1阳性产物主要表达于OPL和IPL。急性HIOP后再灌1天时,与正常组相比,此时GLT-1表达增加(P<0.05)。再灌3天时, GLT-1表达量继续增加。第7天时GLT-1表达开始下调,至14天时,GLT-1表达明显低于3天组,但仍高于正常组(P<0.05)。结论:急性眼高压可导致视网膜GLT-1表达增加,其机制可能与其自身的保护反应有关。Objective: To observe the expression of GLT-1 in the rat retina following acute high intraocular pressure (HIOP). Methods: The intraocular pressure of left eyes in the adult rats were increased to b wave disappearance of flash electroretinogragh and then sustained 60 minutes. After survival for 1,3,7 or14d, the expression of GLT-1 in the rat retina were detected by immunohistochemistry and the cell number in the ganglion cell layer (GCL) were observed by Nissl staining. Results: The cell number in the GCL and the thickness of inner retina were gradually decreased with the increase of survival after HIOP. GLT-1 immunoreacfivity was mainly localized in inner plexiform layer(IPL) and outer plexiform layer(OPL). Compared to the normal control, the GLT-1 expression significantly increased at day 1 after reperfusion.(P〈0.05), and furhter increased at 3 days reperfusion. At day 7 and day 14, the GLT-1 expression reduced gradually but still higher than that in normal control group (P〈 0.05). Conclusion: The results suggest that HIOP can lead to the increase of retinal GLT-1 expression, which is probably related to the retinal protection responses
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