一种新型的适用于延迟溶栓治疗的大鼠脑血栓模型的制作  

A modified thromboembolic stroke model of rat suitable for the delayed thrombolytic therapy

作　　者：尤小芳[1] 李明华[1] 赵俊功[1] 李永东[1] 程英升[1] 

机构地区：[1]上海交通大学附属第六人民医院放射科,200233

出　　处：《介入放射学杂志》2007年第5期330-333,共4页Journal of Interventional Radiology

基　　金：国家自然科学资金资助项目(30470506);上海市科委重点资助项目(034119851)

摘　　要：目的通过对血栓大小的改良,建立适用于延迟溶栓治疗的大鼠脑血栓模型。方法将10个长度0.8～1.0mm、宽度0.35mm的白色血栓经颈内动脉分别注入栓塞脑动脉,形成局灶性脑血栓模型。接受脑血管栓塞的138只SD雄性大鼠被随机分为两大组,分别为单纯缺血组(n=68)和延迟溶栓治疗组(n=70)。单纯缺血组不接受任何治疗;延迟溶栓治疗组分别在血栓注入3、6和9h后,将10mg/kg的rt-PA自股静脉缓慢注入。使用MRI评价梗死灶的位置、大小、栓塞后不同时间的相对脑血流量。末次MRI检查结束后,大鼠脑组织用4%甲醛固定,作病理检查。结果共有131只大鼠在血栓注入后脑内形成明确梗死灶,模型成功率为95%,脑梗死仅位于同侧大脑半球占84.7%(111/131),局限于左侧顶叶皮质或(和)左侧基底节的占79.4%(104/131),局灶性脑梗死体积占同侧大脑半球(23.12±6.04)%;对侧大脑半球也同时出现梗死灶占14.5%(19/131)。发生局灶性出血11只,大面积出血1只,出血率为9.2%(12/131),均发生在延迟溶栓组。单纯缺血组大鼠,在血栓注入后3、6和9h的rCBV分别为(34.13±17.55)%、(40.67±25.91)%和(40.72±26.51)%,各组之间rCBV均无显著差异(分别为3h和6h,Z=-0.958,P=0.338,3h比9h,Z=-1.147,P=0.251)。延迟溶栓后成活率高(24h死亡13只;48h死亡1只,溶栓后8d和12d各死亡1只)。结论改良后的大鼠脑血栓模型是可靠的可重复性的类似于人类大脑中动脉分支栓塞的脑血栓模型,适用于研究延迟溶栓治疗的大鼠脑血栓模型。Objective To develop a modified focal thromboembolic stroke model of rat suitable for the delayed thrombolytic therapy. Methods Ten fibrin-rich autologous blood clots （0.8 - 1.0 mm in length, 0.35 mm in width）were injected into the internal carotid artery to establish a focal thromboembolic stroke model. 138 embolized rats were divided randomly into ischemic group （n = 68）and delayed thrombolytic therapeutic group （n = 70）. At 3 h, 6 h and 9 h after embolization, the rats in delayed thrombolytic therapeutic group were treated with 10 mg/kg recombinant tissue plasminogen activator respectively, while no treatment was performed on isehemie group. Lesion size, location, relative cerebral blood flow volume at different time, and intracranial hemorrhage after treatment were evaluated by MRI. After the last MRI examination, the rats were decapitated and their brain tissue were fixed with formalin for further pathology study. Results Embolization with a pre- formed clot resulted in a focal infarction in the territory supplied by the MCA branch or anterior choroid artery, including 79.4% （104/131） of infarction located in ipsilateral parietal cortex or/and audoputamen, with infarction volume of（23.12 ±6.04）% in embolized hemisphere. At 3 h, 6 h, and 9 h after embolization, the relative eerehral blood flow volumes were （36.86 ± 16.02）%, （40.67 ± 25.91）% and （44.72 ± 26.51）% respectively, with no statistical difference between them（3 h vs 6 h, Z = -0.958, P = 0.338, 3 h vs 9 h, Z=- 1.147, P=-0.251 ）. Hemorrhage was observed in 12 rats, all of that were in delayed thrombolytic therapeutic group. 13 rats died wthin 24 hours and 3 died within 12 days after delayed thrombolyic therapy. Conclusion The modified rat thromboembolic brain model is reliable and repeaducible closely mimicking that of the human middle cerebret urterial branch thrombosis, suitable for invastigating the delayed thrombolytic therapy in rats. （J Intervent Radiol, 2007, 16： 330-333）

关 键 词：脑缺血 血栓栓塞性卒中 大鼠 

分 类 号：R-332[医药卫生] R743