降低内毒素对大鼠肝肺综合征肺损伤的作用  

Reduction of endotoxin may protect lung from injury in rats with hepatopulmonary syndrome

作　　者：王文擘[1] 贾长库[1] 梁靓[1] 朱有法[2] 周元[1] 俞加法[1] 郑权[1] 郑树森[1] 

机构地区：[1]浙江大学医学院附属第一医院卫生部多器官联合移植重点实验室,浙江杭州310003 [2]浙江大学医学院病理教研室,浙江杭州310058

出　　处：《浙江大学学报（医学版）》2007年第3期285-290,共6页Journal of Zhejiang University(Medical Sciences)

摘　　要：目的:探讨氧氟沙星降低内毒素对大鼠肝肺综合征的肺损伤保护作用。方法:结扎SD大鼠胆总管,建立肝肺综合征(HPS)动物模型。将大鼠分成3组(每组10只),假手术组:分离大鼠胆总管,腹腔注射生理盐水2ml;HPS模型组:结扎大鼠胆总管,腹腔注射生理盐水2ml;氧氟沙星组:结扎大鼠胆总管后腹腔注射左旋氧氟沙星(20mg/kg体重)。6周后检测血清内毒素,血、腹水、胆汁培养需氧或厌氧菌,血气分析,测定门静脉压力,肺干重/湿重比,肺灌洗液和肺组织匀浆中髓过氧物酶含量及丙二醛浓度,并评价肺组织病理变化。结果:与HPS模型组比,氧氟沙星组血浆内毒素浓度显著下降[(59±6.2vs268±35.6)ng/L,P<0.01],门静脉压力[(19.28±2.3vs17.80±2.18)cmH2O,P<0.01]、肺组织肺干重/湿重比(5.1±0.7vs4.9±0.7,P<0.01)、肺髓过氧物酶[(0.40±0.10vs0.24±0.03)U,P<0.01]和丙二醛[(0.32±0.05vs0.22±0.03)μmol/L,P<0.01]含量均有显著降低,肺组织炎症改变明显减轻。结论:降低内毒素可以减轻肝肺综合征的肺损伤。Objective： To study the protective effects of ofloxacin by reduction of endotoxin on lung in rats with hepatopulmonary syndrome （HPS）. Methods. Rat models of HPS were induced by ligating the bile duct to result in the biliary cirrhosis. Thirty male SD rats were randomly divided into three groups with 10 animals in each one：sham operation group （the bile ducts were isolated and 2 ml NS was injected i. p） ,HPS model group （the bile ducts were ligated and 2 ml NS was injected i. p） and levofloxacin group （the bile ducts were did as the formmer group and 20 mg/kg ofloxacin injected i. p）. After 6 weeks the portal pressure,ratios of dry weight to wet weight of lung （D/W）, weight of spleen, plasma levels of endotoxin, MPO active and MDA contents in the lung. Plasma and lavage fluid of lung were examined. The results of the blood-gas analysis,the bacterial culture of blood,bile and ascites and the evaluation of pathologic change of lung be also investigated. Results. In levofloxacin group, portal pressure F（19.28 ± 2.3） vs （17.80±2.18）cm H2O, P〈0.01],D/W F（5.1±0.7） vs （4.9±0.7）,P〈0.01],plasma endotoxin levels [-（59±6.2） vs （268±35.6）ng/L,P〈0.01],MPO active F（0.40±0.10） vs （0.24±0.03）U,P〈0.01] and MDA [-（0.32±0.05） vs （0.22±0.03）μmol/L,P〈0.01] contents in the lung were significantly decreased compared with those in HPS group, and the inflammation of lung was also obviously alleviated. Conclusion： Reduction of endotoxin can attenuate the injury of lung in HPS rats.

关 键 词：内毒素类/药理学 氧氟沙星/治疗应用 肝肺综合征/病理学 肝肺综合征/药物疗法 疾病模型 动物 

分 类 号：R575[医药卫生—消化系统]