以CpG为佐剂的人IL-24多克隆抗体的制备  

CpG-ODN is an adjuvant for preparation of the hIL-24 polyclonal antibody

作　　者：黄俊琼[1] 盛伟华[1] 谢宇锋[1] 缪竞诚[1] 杨吉成[1] 

机构地区：[1]苏州大学医学院细胞与分子生物学教研室,江苏苏州215123

出　　处：《现代医药卫生》2007年第11期1583-1585,共3页Journal of Modern Medicine & Health

摘　　要：目的:采用在原核表达系统表达的人IL-24蛋白及人IL-24真核重组质粒免疫新西兰兔,制备兔抗人IL-24多克隆抗体。方法:利用IPTG诱导hIL-24在大肠杆菌中表达,纯化hIL-24重组蛋白,纯化后的蛋白经SDS-PAGE分析,同时提取hIL-24真核重组质粒,用以免疫新西兰兔,并以CpG为佐剂制备多克隆抗体。Western-blot鉴定抗体的特异性,ELISA法测定抗体效价。结果:人IL-24经IPTG诱导后可在大肠杆菌中大量表达,表达量占细菌总蛋白的30%,纯化后的蛋白纯度高;原核表达的重组蛋白和真核重组质粒免疫新西兰兔,通过抗体效价测定,获得了抗血清效价达1∶640的多克隆抗体。结论:原核表达的hIL-24蛋白和hIL-24真核重组质粒均能刺激家兔产生抗体,其多克隆抗体的效价较高。Objective：To immunize rabbits with hIL-24 protein expressed in E.coli and eukaryotic recombinant plasmid to prepare hIL-24 polyclonal antibodies.Methods：The recombinant protein was achieved in E.coli through IPTG induction and was purified. The protein was analyzed by SDS-PAGE. Rabbits were immunized with hIL-24 protein and eukaryotic recombinant plasmid to prepare hIL- 24 polyclonal antibodies.CpG-ODN was used as an adjuvant for preparation of the ML-24 antibody.The antibodies were detected by western-blot and ELISA.Results：Human IL-24 could be expressed in E.coli after IPTG induction. The recombinant protein was about 30% of total expressed products in E.coli. Purified protein was achieved. The polyclonal antibodies were successfully prepared and had higher titter （1：640）. Conclusion： HIL-24 recombinant protein expressed in E.coli and eukaryotic recombinant plasmid can stimulate antibody in rabbits.

关 键 词：人IL-24 原核表达 真核重组质粒 多克隆抗体 CPG 

分 类 号：R33[医药卫生—人体生理学]