血清对联合培养神经上皮干细胞与Schwann细胞的影响  

Influence of serum towards co-cultured neuroepithelial stem cells and Schwann cells

作　　者：暴丽华[1] 李振华[1] 孙晋浩[1] 丛丽[1] 刘文静[1] 高英茂[1] 

机构地区：[1]山东大学医学院解剖学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第5期437-440,445,共5页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2000C14);山东省优秀中青年科学家科研奖励基金资助课题(2004BS02012)

摘　　要：目的:探讨血清对联合培养神经上皮干细胞与Schwann细胞的影响,进一步寻求诱导神经上皮干细胞定向分化的影响因素。方法:采用不同浓度血清联合培养神经上皮干细胞与Schwann细胞,收集上清液作为条件培养液,免疫细胞化学染色观察条件培养液诱导神经上皮干细胞分化,统计分析分化为神经元与星形胶质细胞的比例;MTT检测条件培养液促Schwann细胞存活及增殖的作用。结果:Schwann细胞促进神经上皮干细胞存活和分化,随着血清浓度增加,神经上皮干细胞分化形成的神经元比例减少;神经上皮干细胞及血清均促进Schwann细胞存活和增殖,随血清浓度增加,存活及增殖率增加。结论:无血清或较低血清浓度有利于共培养的Schwann细胞存活和增殖,同时也有利于共培养神经上皮干细胞存活和向神经元方向分化。Objective： To explore the influence of serum of different concentrations towards co-cultured neuroepithelial stem cells and Sehwann cells, and further to seek the factors inducing the orientation differentiation of neural stem cells. Methods： The supematant was collected as a conditional medium from a flask of co-culturing neuroepithelia stem cells and Sehwann cells in serums of different concentrations. The neurons and the astroglia cells differentiated from the neuroepithelial stem cells induced by the conditional medium were counted by the immunocytochemistry technique and the soft IPP. The survival and the proliferation of the Sehwarm cells were determined by MTT. Results： The Sehwarm cells promoted the survival and differentiation of neuroepithelial stem cells, and the number of the neurons decreased with an increase of serum concentration. Both the neuroepithelial stem cells and serum promoted the survival and the proliferation of the Sehwarm cells. Conclusion： Serum of free or lower concentration is favorable for Sehwann cell survival and proliferation and it is advantageous to the survival and differentiation of neuroepithelial stem cells into neurons.

关 键 词：干细胞 神经上皮 分化 雪旺细胞 

分 类 号：R322.8[医药卫生—人体解剖和组织胚胎学]