水杨梅根提取物的体外抗肿瘤活性  

Anticancer Effects of Root of Adina Rubella Hance Extract in Vitro

作　　者：叶勇[1] 涂先琴[1] 宋兴文[1] 施敏荣[1] 江虹[1] 

机构地区：[1]华南理工大学化工与能源学院绿色化学产品技术广东省重点实验室,广州510640

出　　处：《浙江中医药大学学报》2007年第3期372-373,共2页Journal of Zhejiang Chinese Medical University

基　　金：华南理工大学自然科学基金资助项目(322-E5041170);学生研究计划SRP项目(B10-Y13014)~~

摘　　要：[目的]分离筛选水杨梅根中抗直肠癌细胞活性部位。[方法]采用系统溶剂法对水杨梅根水提取物进行分离,应用MTT比色法对分离得到的部位进行体外抗肿瘤活性的测定。[结果]乙酸乙酯提取物对直肠癌LS174T细胞表现出较强的抑制作用,在测定浓度范围内呈现出良好剂量依赖性抑制作用,而正丁醇和氯仿提取部位的抑制作用较弱。[结论]乙酸乙酯提取部位是水杨梅抗肿瘤主要的活性部位。[Objective] To screen the anticancer fraction from extract of root of Adina rubella Hance. [ Methods]To separate the water extract from root of Adina rubella Hance by different solvens,then determine their anticancer activities of the different extracts in vitro with MTT method. [ Results]The ethylacetate extract strongly inhibited cell proliferation of LS174T,and showed significant dosage-dependent response while the butanol extract and the chloroform extract showed low anticancer activities in vitro. [Conclusion]The ethylacetate extract is the principal anticancer extract from root of Adina rubella Hance.

关 键 词：MTT法 水杨梅根 直肠癌 

分 类 号：R285.6[医药卫生—中药学]