非水毛细管电泳/紫外检测法测定肉桂中的肉桂酸和肉桂醛  

Determination of Cinnamaldehyde and Cinnamic Acid in Cinnamon by Non-aqueous Capillary Electrophoresis with UV Detection

作　　者：李利军[1] 黄文艺[2] 冯军[2] 程昊[2] 孔红星[1] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广西大学化学与化学工程学院,广西南宁530005

出　　处：《分析测试学报》2007年第2期274-276,共3页Journal of Instrumental Analysis

基　　金：广西教育厅科研基金资助项目(2000392);广西自然科学基金资助项目(0481019)

摘　　要：建立了用非水相体系高效毛细管电泳/紫外检测法同时测定肉桂酸和肉桂醛的新方法,考察了运行电压、非水相介质和电解质等因素的影响.在25℃下,以乙腈和碳酸丙烯酯（体积比3∶2）的混合液为缓冲体系的溶剂,缓冲体系中含25 mmol/L十六烷基三甲基溴化铵,0.375%（ψ）乙酸,以碳酸丙烯酯为样品溶剂,重力进样30 s,运行电压20 kV,毛细管总长45 cm,有效长度30 cm,ψ5 μm,检测波长310 nm.肉桂酸线性范围为4～100 mg/L,r=0.999 4,检出限为0.80 mg/L,RSD为1.07%.肉桂醛的线性范围10～240 mg/L,r=0.999 6,检出限为2.30 mg/L,RSD为2.19%.应用于肉桂中的肉桂醛和肉桂酸的测定,结果满意.A new method for the determination of cinnamaldehyde and cinnamic acid was established by non-aqueous capillary electrophoresis with UV detection. The effects of the different types of non - aqueous buffer solutions, the concentrations of electrolyte and the applied voltage were investigated. The buffering solution used in this new method was the mixture of acetonitrile and propylene carbonate（3 ： 2, by volume） containing 0. 375% （φ） acetic acid and 25 mmol/L hexadecyltrimethyl ammonium bromide. The applied voltage was 20 kV and the UV detection wavelength was 310 nm. The linear ranges of the method were 10 - 240 mg/L （ r = 0. 999 6 ） for cinnamaldehyde and 4 - 100 mg/L （ r = 0. 999 4 ） for cinnamic acid, respectively. The recoveries of cinnamaldehyde and cinnamic acid were 97% , 104% and the detection limits were 2. 3 mg/L, 0. 80 mg/L, respectively. This method was simple, rapid and accurate. It had been successfully used for the determination of cinnamaldehyde and cinnamic acid in cinnamon.

关 键 词：肉桂 肉桂酸 肉桂醛 非水相体系 毛细管电泳 紫外检测 

分 类 号：O657.8[理学—分析化学] R284.1[理学—化学]