Plexin A1在胃癌中的表达及其与肿瘤细胞增殖和血管生成的关系  

Expression of Plexin A1 in gastric carcinoma and its relationship with tmnor angiogenesis and proliferation

作　　者：赵向阳[1] 陈凛[1] 许倩[2] 李玉红[3] 

机构地区：[1]解放军总医院普通外科,北京100853 [2]承德医学院基础医学研究所 [3]承德医学院病理教研室

出　　处：《中华胃肠外科杂志》2007年第3期265-268,共4页Chinese Journal of Gastrointestinal Surgery

基　　金：国家自然科学基金资助项目（30570522）

摘　　要：目的探讨Plexin A1在胃癌组织中的表达及其与肿瘤细胞增殖和血管生成的关系。方法应用RT．PCR检测20例胃癌患者的癌组织及胃切缘正常胃黏膜组织Plexin A1的mRNA表达：免疫组织化学方法检测50例胃癌组织和20例胃正常黏膜中Plexin A1、肿瘤细胞增殖指数Ki-67、血管内皮生长因子（VEGF）和第Ⅷ因子微血管密度（MVD）的表达。结果RT-PCR示胃癌组织中的Plexin A1 mRNA的表达明显高于胃正常黏膜（0．71±0．37vs0．60±0．25，P〈0．05）。胃癌组织中MVD随着Plexin A1的mRNA表达的增强（r=0．8736，P〈0．01）和蛋白表达的增高而增高（P〈0．01）；Ki-67的表达也与Plexin A1存在明显的一致性（r=0．4851，P〈0．01）；而VEGF的表达与Plexin A1的表达无关。结论Plexin A1在胃癌发生发展中发挥重要作用．与调节血管生成和促进肿瘤细胞增殖有关。Objective To investigate the expression of Plexin A1 in gastric cancer（GC） and its relationship with angiogenesis and proliferation of GC. Methods Plexin A1 mRNA expression in 20 cases of GC specimens and matched resected normal gastric tissue were examined by RT-PCR. Plexin A1, Ki-67, vascular endothelial growth factor（VEGF） protein expression and microvessel density （MVD） in 50 cases of GC specimens and 20 cases of normal gastric mucosa were detected by immunohistochemistry. Results RT-PCR showed that the expression of Plexin A1 in GC was significantly higher than that in normal gastric mucosa （0.71±0.37 vs 0.60±0.25,P 〈 0.05）. MVD within tumor tissue increased significantly with Plexin A1 mRNA expression（r=0.8736,P 〈 0.01） and Plexin A1 protein expression（P 〈 0.01）. The Plexin A1 expression was positively correlated with the proliferation index （Ki-67） of cancer cells（r=0.4851,P 〈 0.01）. However, the Plexin A1 expression level had no relationship with the VEGF expression level. Conclusion Plexin A1 plays an important role in the occurrence and development of GC. Plexin A1 may regulate angiogenesis, and promote the proliferation of GC cells.

关 键 词：受体 Plexin A1 胃肿瘤 肿瘤细胞增殖 血管生成 

分 类 号：R735[医药卫生—肿瘤]