PCR-DGGE对长江河口八种野生鱼类肠道菌群多样性的比较研究  

PCR-DGGE analysis of bacterial diversity of the intestinal system in eight kinds of wild fishes from the Changjiang river estuary

作　　者：李可俊[1] 管卫兵[2] 徐晋麟[3] 张延[1] 赵立平[1] 

机构地区：[1]上海交通大学生命科学技术学院分子生态及生态基因组学实验室,上海200240 [2]上海水产大学海洋科学与技术学院,上海200090 [3]上海交通大学生命科学技术学院,上海200240

出　　处：《中国微生态学杂志》2007年第3期268-269,272,共3页Chinese Journal of Microecology

摘　　要：目的分析长江河口捕获的8种野生鱼类的肠道菌群多样性的差异并观察这种差异与食性的联系。方法采用PCR-DGGE(denaturing gradient gel electrophoresis)技术,DGGE图谱用PCA(principal component analy-sis)方法进行分析。结果建立了长江口8种鱼野生条件下肠道菌群的DGGE指纹图谱,观察到它们在野生条件下的肠道菌群的差异。其中,营底栖生活的舌鰕虎鱼的肠道菌群和其他7种野生鱼有着明显的差异,其他7种鱼的肠道菌群多样性的差异与它们的食性差异相关。结论PCR-DGGE技术是一种能够快速有效地分析研究鱼类肠道菌群结构的技术。8种野生鱼的肠道菌群的结构有明显的差别。Objective To investigate the diversities of intestinal bacteria in eight kinds of fishes from the Changjiang river estuary. Methods PCR-DGGE（ denaturing gradient gel electrophoresis）and PCA （principal com-ponent analysis） were used. Results It was proved that there were abundant and various bacteria in the guts of eight kinds of fishes. The bacterial community structures in the guts of these fishes were obviously different. Conclusions PCR-DGGE analysis of 16S rRNA gene were proved to be a powerful tool for gaining detailed insight into the bacterial diversity of the intestinal system in fish. The correlation between the intestinal bacteria in fish and feed were obvious.

关 键 词：鱼类 肠道菌群 PCR—DGGE 16S RRNA 

分 类 号：R378[医药卫生—病原生物学]