饮料中棒曲霉素和5-羟甲基糠醛的液相色谱-质谱测定方法  

Determination of patulin and 5-Hydroxymethylfurfural in beverage by liquid chromatography-mass spectrometer

作　　者：王云凤[1] 常春艳[1] 葛宝坤[1] 王志英[1] 黄国明[1] 

机构地区：[1]天津出入境检验检疫局,天津300456

出　　处：《中国卫生检验杂志》2007年第5期841-842,共2页Chinese Journal of Health Laboratory Technology

摘　　要：目的:建立一种测定饮料中棒曲霉素和5-羟甲基糠醛的LC-MS方法。方法:样品经固相萃取柱净化,液相色谱柱分离,选择离子监测(SIM)质谱定量测定。结果:棒曲霉素和5-羟甲基糠醛分别在0.01-0.5 mg/L和1-50.0 mg/L范围内线性关系良好,相关系数为0.9973和0.9997,方法的检测低限分别为0.01 mg/L和1 mg/L。两种物质的平均回收率为89.0%-94.0%,重复7次实验RSD值小于12.9%。结论:该方法具有较高的灵敏度、准确性和精密度,可满足饮料中棒曲霉素和5-羟甲基糠醛分析的要求。Objective：To develop a method for analyzing Patulin and 5 -Hydroxymethylfurfural by liquid chromatography - mass speertometer. Methods：The sample was cleaned with solid phase extraction,seperated with LC and determined by MS/ SIM. Results：The calibration curves of standard solutions were linear in the ranges of 0. 01 - 0. 5 mg/L for patulin, 1 - 50 mg/L for 5 -Hydroxymethylfurfural,relative coefficient was over 0. 9973 ,the average recoveries of two analytes ranged from 89. 0% to 94. 0% ,pricisions（RSD） were under 12. 9% ,with limits of detection of 0. 01 mg/L and 1 mg/L respectively. Conclusion：The method is simple, rapid, accurate and suitable for the determination of patulin and 5 -Hydroxymethylfudural in beverage.

关 键 词：饮料 棒曲霉素 5-羟甲基糠醛 固相萃取 液相色谱-质谱 

分 类 号：O658[理学—分析化学]