PCR检测技术在志贺菌引起的腹泻疫情中的应用  

Application of PCR detecting technique for rapid diagnosis of bacillary dysentery

作　　者：贺电[1] 张晓曦[1] 单志兰[1] 曾照丽[1] 白瑞婷[1] 王晨[1] 韩庆华[1] 

机构地区：[1]北京市朝阳区疾病预防控制中心,北京100021

出　　处：《中国卫生检验杂志》2007年第5期858-860,共3页Chinese Journal of Health Laboratory Technology

基　　金：朝阳区组织部优秀青年知识分子人才资助项目

摘　　要：目的:通过采用PCR方法对某次疫情中分离的福氏1 a志贺菌进行保守基因检测及毒力基因分析研究,探讨在感染性腹泻疫情中,PCR技术快速检测志贺菌的应用。方法:对标本中分离的7株福氏1 a志贺菌,用PCR方法检测侵袭性质粒抗原基因(ipaH)、志贺肠毒素1(ShET-1)和志贺肠毒素2(ShET-2)的基因检测,并进行基因分型。结果:7株F1 a菌均检测出ipaH基因,ShET-1和ShET-2,基因型为ShET1(+)/ShET2(+),整个实验可在3 h内完成。结论:该所建立的PCR检测方法准确、快速、简便、特异性强,为其应用于环境、食品及临床腹泻样本中志贺菌的检测打下良好基础。ShET-1/ShET-2基因分型对福氏志贺菌暴发流行中的同源性分析有重要意义。Objective：To explore the PCR rapid detection of Shigella spp. in bacillary dysentery by PCR assay of the conserved gene and toxin gene of the Shigella flexneri 1 a isolated from a dysentery samples. Methods： Seven strains isolated from the dysentery were detected by PCR technique with the invasive plasmid antigen H （ipaH） gene, enterotoxin 1 （ ShET - 1 ） and 2（ ShET - 2）. Results：All the strains were ipaH positive and were found to produce ShET - 1 and ShET -2. The assay could be finished in 3 hours. Conclusion：The PCR detection of ipaH, ShET - 1 and ShET -2 gene is a simple and rapid method with protential application in the identification Shigella spp. in the environment, food and clinical samples.

关 键 词：PCR 志贺菌 快速检测 志贺肠毒素 

分 类 号：R155.31[医药卫生—营养与食品卫生学]