阿司匹林调节小鼠移植性肝癌的基因表达  

Role of aspirin in regulating the gene expression of hepatoma transplanted into mouse

作　　者：赵勇[1] 章必成[1] 朱宇泽[1] 欧武陵[1] 饶智国[1] 高建飞[1] 杜光祖[1] 

机构地区：[1]广州军区武汉总医院肿瘤科,湖北武汉430070

出　　处：《第四军医大学学报》2007年第10期900-902,共3页Journal of the Fourth Military Medical University

摘　　要：目的观察阿司匹林调节小鼠H22移植性肝癌细胞血管内皮生长因子(VEGF)和p53的表达,探讨该药抑制肿瘤的作用机制.方法昆明种雄性小鼠60只,按随机分组为A^F6组;A^E组于前肢右腋皮下接种H22肿瘤细胞悬液(1×1010/L)0.12mL,24h后灌胃给药.A^C组分别给予80g/L,40g/L,20g/L阿司匹林1mL,D组予20g/L5-FU1mL,E组予生理盐水1mL,每日1次,连续用药10d,停药24h,称质量并处死动物,剥取瘤块称质量,计算出肿瘤生长抑制率,用免疫组化法检测癌细胞中VEGF和p53蛋白的表达.F组予饲料及水分正常饲养.结果A,B,C,D组均有明显的抑瘤作用(分别为49.84%,23.89%,29.07%,54.63%),其中以A组和D组的抑瘤作用最明显(与B,C组比较P<0.05).造模各组动物的肝癌细胞中均见VEGF和p53阳性染色,但阳性表达程度不同,与B,C,E组比较,阿司匹林大剂量(A)组和5-FU(D)组可降低肝癌细胞中VEGF,p53的阳性表达(P<0.05).结论阿司匹林对小鼠H22移植性肝癌有抑瘤作用,抑制肿瘤生长的机制可能与下调VEGF和p53的水平有关.AIM： To observe the regulating effects of aspirin on the expressions of vascular endothelial growth factor （VEGF） and p53 in the hepatocareinoma 22 （ H22 ） transplanted into mice, and to explore its tumor-inhibiting mechanism. METHODS： Sixty Kunming male mice were randomly divided into 6 groups （A, B, C, D, E, F） . Then 0. 12 mL H22 cell suspension （ 1 × 10^10/L） was injected to each mouse in the first 5 groups by right axillary subcutaneous vaccination in the forefoot. After 24 h, the mice were treated with medicine according to their weights. Group A, B and C were given 80, 40, 20 g,/L of aspirin, respectively, while group D 5-FU （20 g/L, 1 mL） and group E normal saline （1 mL）. All the medicine was given through gavage, 1 time/ day. The treatment course lasted for 10 d. Then the medicine was withdrawn, and 24 h later the mice were weighed and killed to get their transplanted tumors to weigh and calculate the inhibitory rate of the tumor. Meanwhile, the expressions of VEGF and p53 were determined by immunohistoehemical method. During the whole process, the group F was raised by normal forage and water. RESULTS： The obvious inhibition of tumor was indicated in group A, B, C and D （49.84%, 23.89%, 29.07%, 54.63%, respectively） with the group A and D having the most obvious effect （ P 〈 0.05 vs group B, C ）. The positive expressions of VEGF and p53 were found in all the model groups with different degree. When compared with group B , C and E , the positive expressions of VEGF and p53 were lower in group A and D distinctively as compared with those in group B, C and E （P 〈 0.05, respectively ） . CONCLUSION： Aspirin has the inhibitory action for transplanted H22 in mice, and its mechanism of inhibiting the tumor growth may have a correlation with the downregulation of VEGF and p53.

关 键 词：阿司匹林 H22 癌 肝细胞 血管内皮生长因子类 基因 p53 

分 类 号：R735.7[医药卫生—肿瘤]