拮抗菌B-FS06的鉴定及其发酵产物对黄曲霉的抑制作用  

Identification of B-FS06 and the Antagonistic Activity of Its Cultural Productions against Aspergillus flavus

作　　者：章挺[1] 胡梁斌[2] 王飞[2] 程罗根[1] 石志琦[2] 

机构地区：[1]南京师范大学生命科学学院,南京210097 [2]江苏省农业科学院食品质量安全与检测研究所,南京210014

出　　处：《中国生物防治》2007年第2期160-165,共6页Chinese Journal of Biological Control

基　　金：江苏省国际科技合作项目(BZ2005045);江苏省农业科学院基金(6110505)

摘　　要：从油菜地分离到一株细菌B-FS06,其表现出对黄曲霉的拮抗作用。根据该细菌的理化性质、微观结构及16S rDNA序列的进化树分析,鉴定菌株B-FS06为枯草芽孢杆菌。细菌发酵液经20%、40%、60%、80%硫酸铵饱和度分级沉淀均表现出抑制黄曲霉活性。采用96孔板法测得该菌胞外蛋白粗提液对黄曲霉的最低抑菌浓度为31.2μg/ml。蛋白粗提液经121℃高压灭菌20min后对黄曲霉抑制率为91.3%。B-FS06 isolated from the rape field was showed to be antagonistic against Aspergillus flavus, and was identified as Bacillus subtilis according to the physiological and biochemical properties, microstructure and the phylogenetic analysis of the 16S rDNA. The antifungal activity against A. flavus was found in each fraction of extracellular crude protein extract （CPE） obtained from precipitation separation with 20 %, 40 %, 60 %, 80 % ammonium sulfate. Assay using 96-well plate illustrated that the minimum inhibitor concentration （MIC） of the CPE against A. flavus was 31.2 μg/ml. After treatment at 121 ℃ for 20 min, the CPE gave 91.1% inhibition of growth against A. flavus.

关 键 词：黄曲霉菌 拮抗细菌 鉴定 抑制作用 

分 类 号：S379.5[农业科学—农产品加工] TS201.3[农业科学—农艺学]