氯氰菊酯降解菌的分离鉴定及其降解特性研究  

Isolation,identification and characterization of a cypermethrin-degrading strain J-2

作　　者：梁卫驱[1] 刘玉焕[2] 李荷[2] 

机构地区：[1]东莞市农业科学研究中心,广东东莞523086 [2]中山大学生命科学学院,广东广州510275

出　　处：《广东药学院学报》2007年第2期199-202,204,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省自然科学基金资助项目(031618);广东省科技计划重点资助项目(C32402)

摘　　要：目的分离得到能高效降解氯氰菊酯的菌株。方法采用选择性培养基从农药厂的污泥中进行富集和分离筛选高效菌株,并且用16S rDNA序列分析法和其生理生化特征对其进行鉴定。结果该菌株鉴定为克雷伯氏菌属(Klebsiella sp.),命名为Klebsiella sp.J-2(登录号为AY989899),菌株J-2能以氯氰菊酯为唯一碳源进行生长,降解氯氰菊酯的最适温度是35℃,最佳pH是7.0。结论菌株J-2是一株能高效降解氯氰菊酯的菌株。Objective To isolate a bacterial strain J-2 capable of degrading cypermethrin from sludge of a pesticide manufacturer. Methods Adopting selective culture to screen and isolate the cypermethrin-degrading strain from the samples and identifying the strain by using the 16S rDNA sequence analysis and its taxonomic characteristics. Results This bacterial strain was identified as Klebsiella sp. named J-2. It could grow with cypermethrin as sole source of carbon . The optimal temperature and pH of cypermethrin degradation by the organism were 35 ℃ and 7.0. Conclusion The bacterial strain J-2 effectively degrade cypermethrin.

关 键 词：氯氰菊酯 克雷伯氏菌 生物降解 

分 类 号：X172[环境科学与工程—环境科学]