鸡免疫球蛋白λ轻链绿色荧光蛋白重组分子在COS7细胞的分泌表达  

Secretable Expression of Recombinant Chicken Igλ/GFP in COS7 Cells

作　　者：张红[1] 刘钧珂[2] 张改平[3] 宋海涛[1] 王青[3] 段艳华[1] 赵光辉[1] 

机构地区：[1]河南农业大学,河南郑州450002 [2]河南教育学院,河南郑州450003 [3]河南省农业科学院,河南郑州450002

出　　处：《河南农业大学学报》2007年第2期204-206,241,共4页Journal of Henan Agricultural University

基　　金：国家自然科学基金重点项目(30230270)

摘　　要：将鸡Igλ轻链信号肽与pEGFP-C1载体的绿色荧光蛋白N端融合产生带有信号肽的中间载体pEGFP-C1-SP,通过对鸡Igλ轻链基因的定向克隆,构建了带有纯化标签的鸡Igλ轻链绿色荧光蛋白真核表达载体.转染COS7细胞后,荧光显微镜观察及Western blotting检测均证明融合蛋白在COS7细胞的分泌表达.The signal peptide of chicken Igλ. light chain was fused to the GFP N terminus, pEGFP-CISP, the vector with signal sequence was then constructed. After double enzyme cutting, the chicken Igλ gene was inserted into eukaryotic expression plasmid pEGFP-CI-SP. The results revealed that the plasmid with fusion gene of chicken Igλ and green fluroscent protein （GFP） was successfully constructed. The constructed recombinant vector was transiently transfected into COS7 cells via lipofectamin,and the secretable expression was observed under fluroscent microscope and in Western blotting. These findings provide a basis for further study of chicken Igλ. functions.

关 键 词：鸡Igλ轻链 绿色荧光蛋白 COS7细胞 分泌表达 

分 类 号：Q864[生物学]