PC-1基因表达增强C4-2B前列腺癌细胞生存  

Over-expression of PC-1 Gene Increases Survival Ability of C4-2B Prostate Cancer Cell Line

作　　者：李素萍[1] 张惠[1] 王健[1] 施庆国[1] 黄翠芬[1] 陈亮[2] 夏令[2] 郭迟鸣[2] 周建光[1] 

机构地区：[1]军事医学科学院生物工程研究所,北京100850 [2]厦门大学生命科学学院细胞生物学与肿瘤细胞工程教育部重点实验室,厦门361005

出　　处：《中国生物化学与分子生物学报》2007年第5期388-393,共6页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金资助(No.30070296)~~

摘　　要：建立稳定表达外源PC-1基因的人前列腺癌骨转移C4-2B细胞模型,初步探讨PC-1基因表达对前列腺癌发展的影响.通过脂质体介导的方法,将融合PC-1基因的真核表达载体pcDNA3·1-PC-1稳定转染C4-2B细胞,Western印迹和RT-PCR技术,分别从蛋白水平和RNA水平确定外源PC-1基因表达.MTT和软琼脂集落形成能力等一系列方法,研究PC-1基因的功能,RT-PCR和实时定量PCR检测前列腺癌发生发展相关基因表达的变化.结果表明,PC-1基因的高表达能够诱导雄激素受体(AR)调控基因和一系列重要的信号通路成员基因PSA、PSMA、NKX3·1、Jagged1、EphA3、SGEF和NOTCH3等表达发生变化.实验结果初步证明,PC-1基因表达在晚期前列腺癌中,以及在雄激素非依赖的转变中可以发挥作用,PC-1基因表达可调控一些重要信号通路.对PC-1基因功能深入研究将有可能为发现新的前列腺癌的诊断治疗分子靶标提供线索.To investigate the potential role of PC-1 gene over-expression on prostate cancer progression, a metastasis and androgen independent prostate cancer cell line C4-2B was used, cell clones constitutively expressing PC-1, and their mock-transfected counterparts were constructed. The expression of ectopic PC-1 was analyzed by Western blot and RT-PCR assay. MTY and colony anchor-independent growth in soft agar were performed to evaluate the effects of PC-1 gene expression on prostate cancer progression. RT-PCR and realtime PCR were used to analyse the expression of differential genes between C4-2B-PC-1 and C4-2B-neo cell lines. Our results showed that stable expression PC-1 induced transcription change of some genes, including PSA , PSMA , NKX3.1, Jaggedl , EphA3, SGEF and NOTCH3, which were the androgen receptor, regulated genes and important signal transduction pathway molecules. These observations indicated that PC-I over- expression might play important role in advanced prostate cancer progression, promote prostate cancer cell androgen independent growth, and may regulate multiple signal transduction pathways. Further study of PC-1 gene function may provide clue to discover new molecular targets for prostate cancer diagnosis and treatment.

关 键 词：C4-2B 细胞 PC-1 基因 基因表达 前列腺癌 稳定转染 

分 类 号：Q71[生物学—分子生物学]