短暂性局灶性脑缺血再灌注后氯化锂对大鼠海马CA1区神经元凋亡及糖原合成酶激酶-3β的影响  

Effects of lithium chloride on neuronal apoptosis and expression of glycogen synthase kinase(GSK)-3beta of hippocampal CA1 region after transient focal cerebral ischemia/reperfusion in rats

作　　者：王新河 王斌[2] 钱燕宁[1] 

机构地区：[1]南京医科大学第一附属医院麻醉科,江苏南京210029 [2]南京医科大学药理学教研室,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第5期407-410,F0002,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家人事部出国留学人员基金资助项目(ZA0001);南京医科大学创新基金资助项目(MC0005)

摘　　要：目的:观察氯化锂对短暂局灶性脑缺血再灌注大鼠海马CA1区神经元凋亡及GSK-3β的影响。方法:SD大鼠126只,随机分为正常(NO)组、假手术(SH)组、缺血再灌注(IR)组、氯化锂1(Li1)组、氯化锂2(Li2)组、氯化锂3(Li3)组。Hoechst染色观察海马CA1区神经元凋亡的变化,免疫荧光染色观察海马CA1区GSK-3β及p-GSK-3β(Ser9)的变化。结果:与SH组比较IR组细胞凋亡明显(P<0.01)。与IR组比较各氯化锂组凋亡细胞明显减少(P<0.05或P<0.01),氯化锂剂量越大,凋亡细胞减少越明显(P<0.05或P<0.01)。各组GSK-3β阳性反应颗粒数差异无显著性。与SH组相比IR组p-GSK-3β(Ser9)明显增多(P<0.01)。与IR组比较各氯化锂组p-GSK-3β(Ser9)明显增多(P<0.01),氯化锂剂量越大,增多越明显(P<0.05或P<0.01)。结论:氯化锂能剂量依赖性的减轻大鼠短暂性局灶性脑缺血后海马CA1区神经细胞凋亡,增加海马CA1区p-GSK-3β(Ser9)表达。Objective：To observe the effect of lithium chloride on neuronal apoptosis and GSK-3β of hippocampal CAI area after transient focal cerebral ischemia/reperfusion in rats. Methods： 126 male SD rats were randomly divided into normal group （NO group）,sham operation group（SH group）,ischemia/reperfusion group（IR group）, Li1 group, Li2 group,and Li3 group. The change of neuronal apoptosis in the CA 1 region was examined by Hoechst staining. The expression and distribution of GSK-3β and p-GSK-3β （Serg） in the cell was examined by immunofluorescence analysis. Results：The apoptotic nuclei could be detected significantly in IR groups（compared with SH group, P 〈 0.01 ）. Compared to IR group, Li groups dose dependently（ 1-3 mmoL/kg） reduced the apoptotic nuclei in happocampal CAI area. There was no visible difference among the total amounts of GSK-3β in each group （P 〉 0.05）. P-GSK-3β （Serg）increased significantly after reperfusion （P 〈 0.01 ）. Lithium chloride treatment can dose dependently increase the expression of p-GSK-3β（Serg）（compared with IR groups, P 〈 0.01 ）. Conclusion： Lithium chloride can dose dependently suppress neuronal apoptosis and GSK-3β expression after MCAO/reperfusion.

关 键 词：脑缺血 细胞凋亡 氯化锂 GSK-3β 大脑中动脉闭塞 

分 类 号：R743.31[医药卫生—神经病学与精神病学] Q74[医药卫生—临床医学]