两种铂类药物对人胃癌细胞Livin基因表达的影响  

Effects of two platinum compounds on the Livin gene expression of gastric cancer

作　　者：刘凌翔[1] 朱华云[1] 张锦英[1] 葛红梅[1] 王同杉[1] 刘平[1] 

机构地区：[1]南京医科大学第一附属医院肿瘤科,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2007年第5期415-418,435,共5页Journal of Nanjing Medical University(Natural Sciences)

基　　金：江苏省"135"医学重点人才(RC2001-052)

摘　　要：目的:研究奥沙利铂(L-OHP)或顺铂(CDDP)对人胃癌细胞株BGC-823生长的抑制作用以及Livin基因表达的变化。方法:应用MTT比色法检测细胞24、48、72h的增殖抑制率,选择3个时间段的不同浓度(IC10、IC30、IC50)铂类药物作用于BGC-823细胞,RT-PCR法检测Livin基因表达,Western-blots法检测Livin蛋白表达量。结果:两种铂类药物作用后,BGC-823的增殖受到抑制,呈时间和剂量依赖性,L-OHP的量效变化更明显。两种铂类药物早期均可抑制Livin表达,CDDP作用细胞72h后Livin表达量开始增加,而L-OHP组仍呈下降趋势。结论:L-OHP是Livin的潜在抑制剂,其对BGC-823的增殖抑制量效变化比CDDP更明显,两者对Livin表达的不同影响将为探讨Livin的调控机制和功能研究提供一定依据。Objective：To investigate the growth inhibition of Oxaliplatin （L-OHP） or Cisplatin （CDDP） in vitro and Livin gene expression induced by the two platinum compounds on human gastric cancer cell line （BGC-823）. Methods：The proliferation inhibition ratio was evaluated by MTT in the time of 24, 48 and 72 hours. Different inhibiting concentrations （IC10, IC30 and IC50） of the two platinum compounds were added into the medium of BGC-823 cells. The expression of Livin gene was detected by RT-PCR, and the protein of Livin was assessed by Western-blot. Results：The proliferation of BGC-823 was inhibited in a dose-and time-dependent manner after the treatment of CDDP or L-OHP, especially the latter was dose-dependent significantly than the former. The expression of Livin, which was down-regulated by the two platinum compounds in the earlier period, was up-regulated after 72 hours with CDDP other than L-OHP. Conclusion：L-OHP, which can more significantly inhibit the proliferation of BGC-823 in a dosedependent manner than CDDP, is a potent inhibitor of Livin. The different influences on the expression of Livin will contribute to the investigation of regulation and function on Livin.

关 键 词：奥沙利铂 顺铂 LIVIN基因 凋亡抑制蛋白 胃癌细胞 

分 类 号：R735.2[医药卫生—肿瘤]