不同细胞类型对cbf α1调控DSPP基因转录的影响  

The effect of core binding factor α1 on transcriptional regulation of dentin sialophosphoprotein in different cell lines

作　　者：郭婷[1] 曹罡[1] 余擎[2] 肖明振[2] 赵守亮[2] 

机构地区：[1]南京军区南京总医院口腔科,江苏南京210002 [2]第四军医大学口腔医学院口腔内科

出　　处：《临床口腔医学杂志》2007年第5期272-274,共3页Journal of Clinical Stomatology

基　　金：国家自然科学基金资助项目(30271418);南京军区总医院资助项目(2006052)

摘　　要：目的:观察核心结合因子α1(core binding factor α1,cbf α1)对不同细胞中牙本质涎磷蛋白(dentin sialophosphoprotein,DSPP)基因转录调控的影响。方法:选择Hela和小鼠成牙本质细胞样细胞MDPC-23为实验细胞;DSPP基因上游2.6kb片段为启动子。采用瞬时转染、报告基因等方法观察在两种细胞中,cbf α1对DSPP基因启动子启动活性的影响。结果:在MDPC-23及Hela细胞中,pGL3-Enhancer-2.6K与pcDNA3-cbf α1共转染组荧光素酶的表达量均小于pGL3-Enhancer-2.6K与pcDNA3共转染组(P<0.01);在MDPC-23细胞中变化更为明显。结论:cbf α1对DSPP基因的转录调控作用受细胞类型的影响。To study the effect of core binding factor α1 （cbf α1） on transcriptional regulation of mouse dentin sialophosphoprotein （DSPP） gene in different cell lines. Method： Hela cell, MDPC-23 cells and the segment of nt - 2475bp to ＋53bp were chosen. After co-transfected, the cells were measured for luciferase activity using the dual luciferase reporter assay system. Result： The luciferase activity was significantly lower in the group of co-transfected with pGL3-En- hancer-2.6K and pcDNA3-cbf α1 than the group of pGL3-Enhancer-2.6K and pcDNA3 （P〈0.01）. And it was more signifi-cant in MDPC-23 cells than in Hela cells. Conclusion： Cbf α1 could regulate the transcription of DSPP, which was affect-ed by cell type.

关 键 词：核心结合因子Α1 瞬时转染 报告基因 转录调控 

分 类 号：R392.11[医药卫生—免疫学]