梗阻性黄疸大鼠肝细胞线粒体DNA片段缺失的定位研究  

Localization of hepatocyte mtDNA deletion after obstructive jaundice in rats

作　　者：唐春[1] 别平[1] 张玉君[1] 李晓武[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,重庆市400038

出　　处：《中华肝胆外科杂志》2007年第4期250-253,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金资助,项目编号（30571806）

摘　　要：目的对梗组性黄疸大鼠线粒体DNA片段缺失进行定位研究，为进一步研究梗阻性黄疸大鼠线粒体功能及肝功能的变化情况打下基础。方法48只健康wistar大鼠随机分为SHAM组（假手术组），BDO组（梗阻性黄疸组），利用大片段PCR结合ApaⅠ及SauⅠ限制性酶切分析及基因测序方法，对大鼠梗阻性黄疸mtDNA的片段缺失状况进行定位研究。结果大鼠梗阻性黄疸肝细胞mtDNA的片段缺失位点位于4101-15294之间。结论大鼠梗阻性黄疸肝细胞mtDNA存在着4101-15294碱基之间约11194bp的片段缺失。Objective To study the positioning of the hepatocyte mitochondrial DNA deletion in obstructive jaundiced rats. Methods Forty-eight Wistar rats were randomized into two groups, namely SHAM, BDO. Positioning study of the hepatocyte mitochondrial DNA deletion in obstructive jaundiced rats was detected by long and accurate PCR, restricted enzyme （Apa Ⅰ and Sau Ⅰ ） and gene sequencing. Results The deletion fragment of hepatocyte mitochondrial DNA locates between 4101 and 15 294 bp in obstructive jaundiced rats. Conclusions About 11 194 bp deletion fragment which locates between 4101 and 15 294 bp of hepatocyte mitochondrial DNA is identified in obstructive jaundiced rats.

关 键 词：梗阻性黄疸 线粒体DNA 片段缺失 

分 类 号：R686[医药卫生—骨科学]