抑癌基因WWOX在肝外胆管癌中的表达  

Expression of WW domain-containing oxidoreductase gene in patients with extrahepatic cholangiocarcinoma

作　　者：顾军[1] 王梅[2] 王雅杰[2] 李咏梅[2] 袁杨[2] 薛春燕[2] 

机构地区：[1]中国人民解放军第123医院肿瘤科,安徽省蚌埠市233015 [2]第二军医大学附属长海医院肿瘤科,上海市200433

出　　处：《世界华人消化杂志》2007年第11期1302-1305,共4页World Chinese Journal of Digestology

基　　金：上海市科委自然科学基金;No.04ZR14003~~

摘　　要：目的:检测WWOX mRNA及其编码蛋白在肝外胆管癌中的表达,分析其临床病理学意义.方法:用Real Time RT-PCR方法定量分析21例胆管癌组织WWOX mRNA表达情况,5例肝移植的正常胆管组织作为对照;采用UIP法染色检测相应病理切片的蛋白表达情况,并比较mRNA和蛋白在不同分级组织中的表达差异.结果:WWOX mRNA在肝外胆管癌中的表达显著低于正常胆管组织(8.936×10^(-7)±3.253×10^(-7) vs 1.079×10^(-6)±1.735×10^(-7),P<0.001),47%表达缺失;蛋白表达的缺失频率为57%,其mRNA及蛋白表达与组织学分级有显著相关(r=-0.583,-0.840,P<0.001),而年龄、性别、术前肝功能及临床分期无显著相关性.低分化胆管癌组织中的表达显著低于分化较好的肿瘤组织(P±<0.05).结论:WWOX表达改变参与胆管癌的发病,可作为预测肝外胆管癌生物侵袭性的有效指标.AIM： To determine the expression of WW domain-containing oxidoreductase （WWOX） messenger RNA （mRNA） and protein, and evaluate its clinicopathological significance in human extrahepatic cholangiocacinoma （ECC）. METHODS： Real-time reverse transcription-polymerase chain reaction and immunohisto-chemistry （UIP method） were used to detect the expression of WWOX mRNA and protein respectively in 21 ECC patients and 5 cases of normal bile duct tissues. The results were comparatively analyzed with the clinicopathological characteristics. RESULTS： The expression of WWOX mRNA was significantly lower in ECC than that in normal bile duct tissues （8.936 × 10^-7±3.253× 10^-7 vs 1.079 × 10^-6＋ 1.735 × 10^-7, p 〈 0.001）, and the loss of mRNA was observed in 47% of the cases （t = 10.145, P 〈 0.001）. Loss of WWOX protein expression was found in 57% of ECC specimens. Both mRNA and protein expression had a significant correlation with the histological grading, and the correlation coefficients were -0.583 and -0.840, respectively. However, WWOX expres- sion had no correlations with other clinicopathological factors such as age, sex, clinical staging or the status of preoperative hepatic function （P 〉 0.05）. Poorly differentiated ECC had markedly lower expression of WWOX than moderately or well differentiated one （P 〈 0.05）. CONCLUSION： Alterations of WWOX expression may be involved in the tumorigenesis of cholangiocarcinoma, which may serve as an indicator for predicting the invasion of ECC.

关 键 词：肝外胆管癌 WWOX 实时RT—PCR 免疫组织化学 

分 类 号：R735.8[医药卫生—肿瘤]