吸烟致口腔癌发生分子机制的研究  

Study on the genetic toxic effect of smoking on human oral mucosa

作　　者：肖灿[1] 周建华[2] 惠建华[1] 李卫东[1] 

机构地区：[1]江苏苏州大学附属第一医院,215006 [2]苏州大学放射医学与公共卫生学院,215006

出　　处：《实用口腔医学杂志》2007年第3期325-328,共4页Journal of Practical Stomatology

基　　金：苏州大学青年教师基金资助(编号:Q3122402)

摘　　要：目的:研究吸烟对口腔黏膜细胞的遗传毒性,分析与口腔癌发生的关系。方法:采用单细胞凝胶电泳技术,检测24例吸烟者和17例非吸烟者(其中口腔恶性肿瘤12例,良性肿瘤19例,10例健康者)口腔黏膜脱落上皮细胞DNA损伤,按组织病理类型进行分析,观察指标为彗星尾长和彗星率。试验所得数据由SPSS、SAS软件进行统计检验和分析。结果:恶性肿瘤患者的彗星尾长和彗星率均明显高于良性肿瘤患者和健康人,有显著性差异(P<0.01),良性肿瘤患者与健康者的彗星试验结果无显著差异(P>0.05),吸烟者的彗星尾长和彗星率均明显高于非吸烟者(P<0.01)。结论:吸烟可导致口腔黏膜细胞DNA损伤,随肿瘤组织病变程度的加重,DNA损伤程度亦加重。组织细胞DNA损伤与口腔恶性肿瘤的发生密切相关。Objective： To study the genetic toxic effect of smoking on human oral mucosa,and the DNA damage of exfoliated oral mucosa cells. Methods：The DNA damage of exfoliated oral mucosa cells was investigated by single cell gel electrophoresis （SCGE） in 12 cases of malignant tumor, 19 cases of benign tumor and 10 health controls. There were 24 smokers and 17 non-smokers among them. The tail length and frequency of comet cells were used to measure DNA damage. SPSS and SAS software were used for statistical analysis. Results：Malignant tumors had a longer tail length and higher frequency of comet cells than benign tumors and health controls, and the difference was statistically significant （P 〈 0.01 ）. However, there was no significant difference between benign tumors and health controls （P 〉 0.05）. The DNA damage of exfoliated oral mucosa cells in smokers was more serious than that of non-smokers（ P 〈 0.01 ）. Conclusion.Smoking can induce DNA damage of oral mucosa cells, and has an important effect on carcino- genesis. The genesis of oral malignant tumor may have a close relativity with DNA damage of cells.

关 键 词：香烟 口腔肿瘤 DNA损伤 单细胞凝胶电泳 

分 类 号：R739.8[医药卫生—肿瘤]