过量氟对大鼠切牙发育过程中Smad3表达的影响  

The effect of overdose fluoride on the expression of Smad3 in rat incisor

作　　者：罗平平[1] 王强[1] 刘辉[1] 刘天麟[2] 高春娜[1] 武红梅[1] 

机构地区：[1]同济大学附属口腔医院口腔内科,200072 [2]同济大学附属东方医院口腔科

出　　处：《实用口腔医学杂志》2007年第3期377-379,共3页Journal of Practical Stomatology

基　　金：同济大学"十五";"211"工程建设资助项目(1504102011)

摘　　要：目的:研究过量氟对大鼠切牙发育过程中Smad3表达的影响,探讨氟斑牙的发病机制。方法:选择20只Wistar大鼠,随机分成2组:Ⅰ组(蒸馏水组)和Ⅱ组(0.1g/LF-)。饲养8周处死动物,利用免疫组化染色的方法观察过量氟对大鼠成釉细胞及成牙本质细胞中Smad3表达的影响。结果:免疫组化结果经图像分析显示实验组大鼠切牙成釉细胞及成牙本质细胞中Smad3的表达均低于对照组(P<0.01),存在显著性差异。结论:过量氟可能通过抑制Smad3的表达来干扰上皮和间充质之间正常的信号转导,进而使釉质的分化发育受到影响。这有可能是氟牙症发生的细胞内机制之一。Objective：To study the effect of overdose fluoride on Smad3 expression during development course of Wistar rat＇s incisor, and then to explore pathogenesis of dental fluorosis. Methods： 20 Wistar rats（ 10 male rats and 10 female rats ） were randomly divided into two groups. Experiment group was feed with water contained 0.1 g/L fluorin and normal group with distilled water was served as control. After 8 weeks all rats were sacrificed. Immunohistochemistry was used to observe the effect of overdose fluorin on Smad3 expression in rat＇s ameloblast and odontoblast. Results： The immunohistochemistry results demonstrated that in experiment group, Smad3 expression in rat＇s ameloblast and odontoblast was lower than those in control group, and statistic analysis showed there were significant differences between the two groups（ P 〈 0.01 ）. Conclusion ： By restraining Smad3 expression, excessive fluorin interfere normal transduction of TGFβ/Smads signal channel, and retard the development of tooth germ. It＇s one of possibility of the dental fluorosis＇s pathogenesis within the cell.

关 键 词：氟化物 大鼠 SMAD 转化生长因子-Β 

分 类 号：R781.2[医药卫生—口腔医学]