P物质在去下牙槽神经下颌骨骨折愈合中的表达  

Expression of substance P in mandibular callus after inferior alveolar nerve section

作　　者：何海涛[1] 谭颖徽[1] 王建华[1] 徐琳[1] 

机构地区：[1]第三军医大学大坪医院口腔科,400042

出　　处：《实用口腔医学杂志》2007年第3期423-425,共3页Journal of Practical Stomatology

基　　金：重庆市自然科学基金(CSTC;2006BB5053)

摘　　要：目的:探讨下牙槽神经对下颌骨骨折愈合的影响,试分析其可能机制。方法:选用中国大白兔32只,分为实验组(去下牙槽神经下颌骨骨折组)和对照组(保留下牙槽神经下颌骨骨折组),分别在术后第7、14、21、28天处死动物,用免疫组化染色检测P物质(SP)在新生骨痂中的表达,并结合计算机图像分析系统进行半定量分析。结果:失神经骨折组骨痂中SP含量早中期表达弱,晚期表达逐渐增强;对照组骨痂中SP在早期表达较强,到14d尤明显,随后呈逐渐下降趋势,晚期SP表达复又增强;SP在骨痂中表达失神经组明显低于同期对照组(P<0.05)。结论:下牙槽神经参与了早期骨修复和晚期的改建,神经参与骨折愈合的调控与SP有关。Objective：To study the effect of sensory nerve on fracture of mandible and try to find the possible mechanism. Methods：Thirty-two China rabbits were used and divided into two groups for study. They all suffered from a standardized fracture in the left mandible, and half of them with inferior alveolar nerve section （IANS） or without. The callus were stained to observe the expression of substance P（SP） by immunochemistry and analysed by a computerized-graph analysing system. Results：Immunoreactivites of substance P occurred weakly on Day 7 and 14 after operation, and became stronger at late stage in IANS group. On the contrary, stronger immunoreactivites of substance P occurred at the early stage, less on day 21 posttrauma and stronger again on day 28 in control group. The strongest immunoreactivites at each stage occurred mainly in bone marrow on day 28 posttrauma in both groups. There was signifi- cant difference in the expressions of substance P in the callus between the two groups （ P 〈 0.05 ）. Conclusion： Sensory nerve may involve in the process of callus formation at early stage and bone remodeling at late stage. It seems that neural involvement in healing of bone is related to the local concentration of SP in the callus.

关 键 词：P物质 下颌骨骨折 去下牙槽神经 骨折愈合 

分 类 号：R782.4[医药卫生—口腔医学]