口山酮抑制肝星状细胞活化作用及其机制研究  

Effect of demethylbellidifolin on activation of hepatic stellate cells and its underlying mechanisms

作　　者：李金成[1,2] 姜德建[3] 吕冬[4] 谭桂山[3] 谭德明[1] 

机构地区：[1]中南大学湘雅医院感染科,湖南长沙410008 [2]邵阳医专传染病学教研室 [3]中南大学药学院 [4]中南大学护理学院

出　　处：《中国医师杂志》2007年第5期593-596,共4页Journal of Chinese Physician

基　　金：湖南省自然科学基金资助项目（06jj3012）

摘　　要：目的研究去甲基雏菊叶龙胆酮（DMB）对肝星状细胞（HSC）活化的影响及其机制。方法培养大鼠肝星状细胞株HSC-T6,分别加入1、3或10μM DMB孵育12-48 h。细胞组织化学检测α-平滑肌肌动蛋白（α-SMA）表达;酶联免疫吸附试验测定I型胶原的合成;逆转录PCR检测转化生长因子-β1（TGF-β1）和结缔组织生长因子（CTGF）mRNA水平。结果体外培养的HSC-T6表现为激活状态,表达α-SMA和合成I型胶原。处理DMB能显著降低体外培养的HSC-T6表达α-SMA细胞数目和I型胶原的合成,且呈浓度依赖性。而且,DMB能浓度依赖性降低HSC TGF-β1和CTGF mRNA水平。结论DMB能抑制HSC的活化,其作用与抑制TGF-β1-CTGF促纤维化通路有关。Objective To investigate the effects of demethylbellidifolin （DMB） on activation of hepatic stellate cells （HSC） and its underlying mechanism. Methods Rat hepatic stellate ceils line HSC-T6 were cultured and treated with different concentrations （ 1,3 or 10 μM） of DMB for 12 to 48 h. Expression of or-smooth muscle actin （α-SMA） and synthesis of I-type collagens in HSC were determined by immunohistochemistry.and ELISA assay, respectively. Messenger RNA levels of transforming growth factor-β1 （TGF-β1） and connective tissue growth factor （CTGF） were determined using RT-PCR. Results HSC-T6 cultured in vitro showed an activated status, which expressed α-SMA protein and synthesized I-type collagen. Treatment of HSC-T6 with DMB could markedly decrease α-SMA-positive cells ratio and suppress I-type collagens production in a concentration-dependent manner. Moreover, DMB concentration -dependently suppressed the mR- NA levels of both TGF-β1 and CTGF in HSC-T6. Conclusions DMB exhibits the inhibitory effects on the activation of HSC, and such effects may be related to inhibiting TGF-β1-CTGF-dependent pathway.

关 键 词：菊科 肝细胞 星形细胞 肌动蛋白类 转化生长因子Β 

分 类 号：R575[医药卫生—消化系统]