降钙素基因相关肽对局灶性脑缺血再灌注大鼠脑cAMP反应元件结合蛋白mRNA表达的影响  

THE EFFECT OF CGRP ON THE EXPRESSION OF CREB mRNA IN RAT HIPPOCAMPUS AND PARIETAL CORTEX DURING FOCAL CEREBRAL ISCHEMIA AND REPERFUSION

作　　者：张正洪[1] 曲鹏[2] 刘玉丽[2] 张宝辉[2] 方秀斌[2] 

机构地区：[1]郧阳医学院解剖学教研室,十堰442000 [2]中国医科大学基础医学院神经生物学教研室,沈阳110001

出　　处：《解剖学报》2007年第3期282-285,共4页Acta Anatomica Sinica

基　　金：辽宁省自然科学基金资助项目(619019)

摘　　要：目的探讨外源性降钙素基因相关肽(CGRP)对局灶性脑缺血再灌注大鼠海马和顶叶皮质cAMP反应元件结合蛋白(CREB)mRNA表达的影响。方法用线栓法制作大鼠局灶性脑缺血再灌注模型,应用原位杂交和图像分析技术检测大鼠缺血侧海马CA1区和顶叶皮质CREB mRNA表达。结果假手术组右侧海马CA1区和顶叶皮质CREB mRNA有明显表达,缺血再灌注组右侧海马CA1区和顶叶皮质CREB mRNA阳性产物吸光度值减少,CGRP组缺血侧海马CA1区和顶叶皮质CREB mRNA表达的吸光度值比缺血再灌注组增高(P<0.05)。结论CGRP上调局灶性脑缺血再灌注大鼠海马和顶叶皮质CREB mRNA的表达,CGRP对缺血神经元的保护作用可能通过激活CREB的转录与翻译,从而启动一系列信号通路来实现。Objective To investigate the effect of calcitonin gene related peptide （CGRP） on the expression of cyclic AMP response element binding protein （CREB） mRNA in rat hippocampus and parietal cortex during focal cerebral ischemia and reperfusion （I/R） . Methods Focal cerebral ischemia/reperfusion model was induced by occluding of the right middle cerebral artery using the intraluminal suture method. Hybridization in situ experiment was used to detect the expression of CREB mRNA in the ipsilateral hippocampal CA1 region and parietal cortex during different reperfusion periods. The positive product of CREB mRNA was analyzed by image analysis system. Results There was a distinct expression of CREB mRNA in right hippoeampal CA1 region and parietal cortex in sham group. The absorbency of CREB mRNA positive product reduced in I/R group as compared to sham group,while it increased in CGRP group than I/R group （ P 〈 0.05）. Conclusion CGRP increases the expression of CREB mRNA in ischemic neurons of the hippocampus and parietal cortex during focal cerebral ischemia and reperfusion. CGRP＇ s protection of ischemic neuron may be conducted by activating the transcription and translation of CREB and then initiating a series of signal transduction pathways.

关 键 词：脑缺血 降钙素基因相关肽 CAMP反应元件结合蛋白 原位杂交 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学] R322.8[医药卫生—临床医学]