人肝癌细胞RNA电转染至未成熟树突状细胞的扫描电镜观察  

A SCANNING ELECTRON MICROSCOPE STUDY ON THE IMMATURE DENDRITIC CELLS BEFORE AND AFTER THE ELECTRANSFECTION OF HUMAN HEPATIC CANCER CELL RNA

作　　者：战军[1] 唐军民[1] 唐岩[1] 

机构地区：[1]北京大学基础医学院组织学与胚胎学教研室,北京100083

出　　处：《解剖学报》2007年第3期334-338,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(39970380)

摘　　要：目的观察电转染人肝癌细胞RNA前后未成熟树突状细胞(imDCs)的表面形态学变化。方法取人外周血,纯化获取单核细胞后诱导其成为imDCs,然后通过电转染方法将人肝癌细胞RNA导入其内;用7种特异性抗体的免疫细胞化学技术对电转染前后的树突状细胞鉴定后,进行扫描电镜观察。结果电转染人肝癌细胞RNA后imDCs内TERT表达显著增加,其他分子表达无显著改变。电转染前,imDCs多呈圆形、椭圆形或不规则形,体积较单核细胞大,但大小不均,在细胞表面可见长短不一的微绒毛,或/和许多泡状、面纱状或云朵状细胞突起;电转染后,可在imDCs表面见到由于电转染所造成的孔,并有一定数量的细胞融合和细胞死亡,但绝大多数细胞可恢复至正常结构状态。结论电转染打孔可为外源物质(如肝癌细胞RNA或其他肿瘤细胞RNA)导入细胞内提供可行性,并为进一步利用imDCs制作肿瘤疫苗,开展肿瘤的生物治疗提供一种可行方案。Objective To observe the morphologic changes of immature dendritic cells（imDCs） before and after the electransfection of human hepatic cancer cell RNA. Methods Monocytes were purified from human peripheral blood, and induced into imDCs. Then human hepatic cancer ceil RNA was electransfected into monocyte-derived imDCs. ImDCs were identified by the immunocytochemical method with 7 specific antibodies before and after electransfection. These dendritic cells were observed by scanning electron microscopy. Results After electransfection of human hepatic cancer ceil RNA there were few changes of molecule expressions in imDCs. ImDCs were in round, oval and irregular shapes before electransfection. Their sizes were not identical but all bigger than monocytes. There were many protrusions in different shapes which looked like dendrite, or/and bubble, veil cloud on the surface of these imDCs. Although there were some ceil fusions and ceil deaths after electransfection, most imDCs recovered from the damage. Electransfecting human hepatic cancer cell RNA into imDCs would make pores on ceil membrane. Conclusions The pores on ceil membrane make it possible that the exogenous material enters imDCs. This study can prove the possibility of electransfecting human hepatic cancer cell RNA into imDCs to make cancer vaccine, which provides a new way for tumor biologic therapy.

关 键 词：电转染 肝癌细胞RNA 未成熟树突状细胞 扫描电镜 人 

分 类 号：R329.4[医药卫生—人体解剖和组织胚胎学]