人牙周韧带细胞成骨诱导分化后的超微结构观察  

THE ULTRASTRUCTURE OF HUMAN PERIODONTAL LIGAMENT CELLS AFTER THE OSTEOINDUCTIVE CULTURE

作　　者：韩劼[1] 孟焕新[1] 唐军民[2] 唐岩[2] 李盛林[3] 张凤秋[1] 

机构地区：[1]北京大学口腔医学院牙周科,北京100081 [2]北京大学基础医学院组织学与胚胎学教研室,北京100083 [3]北京大学口腔医学院口腔颌面外科实验室,北京100081

出　　处：《解剖学报》2007年第3期343-346,共4页Acta Anatomica Sinica

基　　金：国家"十五;211工程";卫生部临床学科重点项目资助(2004-2006)

摘　　要：目的观察成骨诱导分化培养前后,人牙周韧带细胞超微结构的变化。方法对人牙周韧带细胞进行体外培养,并进行成骨诱导分化,14d后行透射电子显微镜观察。结果体外成骨诱导分化培养后,人牙周韧带细胞内细胞器发达,数目较常规培养条件下明显增多。细胞内可见大量的基质小泡、髓样结构及中、低电子密度的圆形和椭圆形小泡样结构。结论人牙周韧带细胞是一种具有成骨潜能的细胞,经成骨诱导分化培养后,其超微结构具有成骨细胞和成牙骨质细胞的特点。Objective To observe the ultrastructure of human periodontal ligament cells after the osteoinductive culture. Methods Human periodontal ligament cells （hPDLCs） were cultured in the osteoinducdve medium with 10^-7 mol/L dexamethasone, 50 mg/L α-ascorbic acid and 10^-2 mol/L β-glycerolphosphate or in ordinary culture for 14 days. Transmission electron microscopy was used to observe the ultrastructural changes. Results After osteoinductive culture, there were richer mitochondria, endoplasmic reticulum and Golgi complex in the cytoplasm of hPDLCs than that in the ordinary clutured hPDLCs. And lots of myelin like structures and matrix vesicles with middle or low electron density were observed in the hPDLCs cultured with the osteoinductive medium. Conclusion The ultrastructural characteristics of hPDLCs after the osteoinductive culture were similar to those of mineral-forming cells, such as osteoblast and cementoblast.

关 键 词：人牙周韧带细胞 超微结构 成骨诱导分化 体外培养 人 

分 类 号：R78[医药卫生—口腔医学]