超声引导下瘤块注射建立兔VX_2肝肿瘤模型  

Establishing A VX_2 Rabbit Liver Tumor Model by Injecting Tumor Tissue Under The Guidance of Ultrasound

作　　者：杨国华[1] 陈孝平[1] 黄志勇[1] 孙振刚[1] 张万广[1] 关键[2] 罗鸿昌[3] 

机构地区：[1]华中科技大学同济医学院附属同济医院肝脏外科中心,武汉430030 [2]华中科技大学同济医学院附属同济医院放射科 [3]华中科技大学同济医学院附属同济医院超声科

出　　处：《中国普外基础与临床杂志》2007年第3期273-275,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家"十五"科技攻关项目资助(编号:2004BA714B)~~

摘　　要：目的研究超声引导下微创建立兔VX2肝肿瘤动物模型的方法,分析兔VX2肝肿瘤超声、CT及血管造影的影像学特征。方法切除植于兔后腿肌肉内的VX2肿瘤组织并剪成0.5mm见方的块状瘤块,在超声引导下,将瘤块直接注射接种于60只新西兰白兔肝左叶,2周后测定兔肝肿瘤接种成功率,观察研究其超声、CT和血管造影的影像学特征。结果兔VX2肝肿瘤接种成功率为95%(57/60);荷瘤兔平均自然生存时间为(45±8)d。肝肿瘤的超声表现为圆形或类圆形低回声结节,其周边及内部可见较丰富的血流信号;多排螺旋CT平扫肝肿瘤表现为低密度结节影;肝动脉造影显示VX2肝肿瘤富含血管且血管网杂乱,肿瘤显影以周边为主。结论超声引导下穿刺接种制作兔VX2肝肿瘤动物模型操作简便,成功率高,其影像学特征与人原发性肝癌相似。Objective To establish a VX2 liver tumor model in rabbits under guidance of ultrasound mini-invasively, and to evaluate the imaging characteristics of VX2 liver tumor on ultrasound, CT scanning and angiography, respectively. Methods VX2 tumor tissues that were planted intramuscularly in the rabbit＇s hind leg, were resected and cut into small pieces in 0.5 mm×0.5 mm×0.5 mm, and were inoculated into the left lobes of livers in 60 New Zealand rabbits under the guidance of ultrasound. The achievement ratio of the inoculated tumors growing in the rabbit livers were measured, and the imaging characteristics of the tumors on ultrasound, CT scanning and angiography were observed and then were evaluated 2 weeks later. Results The achievement ratio of establishing a liver tumor model under the guidance of ultrasound was 95 % （57/60）. The average physio-life span of the model rabbits was （45±8） d. Ultrasound showed that the tumor in the rabbit liver was a single spherical or sphere-alike hypoechoic nodus, and there were higher blood flow signals in and around the nodus. VX2 tumor in the liver appeared as a solitary low density nodus on unenhanced CT scanning, and the hepatic arteriography showed that the tumor was rich of blood vessels, which built the disordered vasoganglion. Tumor was maily stained at the edge of the nodus. Conclusion It may be simple and effective to estabish a rabbit liver tumor model by inoculating VX2 tumor tissue under the guidance of ultrasound and the achievement ratio of such method was relatiely high. And the imaging characteristics of the tumor was similar to that of human primary liver carcinoma.

关 键 词：肝肿瘤 动物模型 

分 类 号：R735.7[医药卫生—肿瘤] R73-354[医药卫生—临床医学]