^(103)钯放射性支架对Fas基因表达影响及与胆管癌细胞凋亡关系研究  

Effect of ^(103)Pd Radioactive Stent on The Expression of Fas Gene and Its Relation with Apoptosis of Bile Duct Cancer Cells

作　　者：何贵金[1] 于发强[1] 高沁怡[2] 许书河[3] 高红[4] 姜维国[5] 蒋涛[4] 戴显伟[1] 戴朝六[1] 

机构地区：[1]中国医科大学盛京医院肝胆外科,沈阳110004 [2]中国医科大学第一临床学院附院核医学科,沈阳110001 [3]中国原子能研究院同位素研究所,北京100243 [4]中国医科大学盛京医院中心实验室 [5]中国医科大学盛京医院病理科

出　　处：《中国普外基础与临床杂志》2007年第3期284-286,共3页Chinese Journal of Bases and Clinics In General Surgery

基　　金：辽宁省自然科学基金资助项目(项目编号:C067);辽宁省教育厅资助项目(项目编号:05L445);辽宁省科技计划项目(项目编号:2006225001-6)~~

摘　　要：目的探讨103钯(103Pd)放射性支架释放的γ射线对Fas表达的影响以及与胆管癌细胞凋亡的关系及意义。方法培养瓶中胆管癌细胞消化后形成细胞悬液,采用血细胞计数法计数细胞,按1×105/ml的浓度分装到2ml塑料冻存管中。分别设置普通支架组及103Pd支架组,应用TUNEL法和免疫组化染色方法检测γ射线诱导胆管癌细胞凋亡的情况和Fas表达的情况。结果103Pd支架组胆管癌细胞的Fas表达较普通支架组明显增高(P<0.05),且胆管癌细胞出现明显细胞凋亡,凋亡细胞数明显高于普通支架组(P<0.01)。结论Fas表达水平与胆管癌细胞凋亡的发生有关,103Pd放射性支架可能通过增强Fas表达,促进胆管癌细胞凋亡,从而为临床应用103Pd放射性支架治疗胆管癌提供理论依据。To research the effect of γ-radiation released from ^103Pd radioactive stent on the expression of Fas gene and its relation with apoptosis of bile duct cancer cells lines. Methods The cancer cells of bile duct were dissociated into suspension in culture flasks, and the number of cells was counted by hemacytometry. The suspension was then stored in 2 ml freezing tubes in the density of 1 ×10^5/ml. They were set in two stents： general stent （general stent group） and ^103pd radioactive stent （^103pd stent group）. The expression of Fas gene and apoptosis of bile duct cancer cells in general stent group and ^103Pd stent group were analysed with immunohistochemistry technique and TUNEL method, respectively. Results The expression level of Fas gene in ^103Pd stent group was significantly higher than that in general stent group （P〈0.05）, and the number of apoptotic cancer cells in ^103Pd stent group was also significantly more than that in general stent group （P〈0.01）. Conclusion There is a correlation between the expression of Fas gene and the apoptosis of bile duct cancer cells, which means that ^103Pd radioactive stent may increase the expression of Fas gene and promote the apoptosis of cancer cells. It may be helpful for the further study of treatment for bile duct cancer using ^103Pd radiative stent.

关 键 词：^103Pd放射性支架 Γ射线 FAS基因 细胞凋亡 

分 类 号：R735.8[医药卫生—肿瘤]