全反式维甲酸对体外培养的人Tenon囊成纤维细胞增殖及凋亡的影响  

Effect of all trans-retinoic acid on the proliferation and apoptosis of human Tenon′s capsule fibroblasts in vitro

作　　者：戴丽华[1] 李维宁[1] 乔智[1] 

机构地区：[1]山东大学齐鲁医院眼科,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第4期376-379,共4页Journal of Shandong University:Health Sciences

基　　金：山东省卫生厅科研基金资助课题(2001CA1CJB21)

摘　　要：目的:观察全反式维甲酸(ATRA)对体外培养的人Tenon囊成纤维细胞(HTCFs)增殖及凋亡的影响。方法:体外培养HTCFs,采用流式细胞仪检测细胞周期改变、细胞凋亡率、抗凋亡基因bcl-2的水平,琼脂糖凝胶电泳检测细胞DNA,研究不同浓度的ATRA对体外培养的HTCFs增殖及凋亡的影响,同时与丝裂霉素C(MMC)作比较。结果:用ATRA和MMC处理后,HTCFs细胞周期的分布发生了明显的变化,表现为G0/G1期细胞数增多,S期细胞数减少;1×10-9mol/L的ATRA即可诱导细胞的凋亡,随着浓度的增加,凋亡率显著增高;ATRA组bcl-2的水平比空白对照组大约低了一倍;DNA琼脂糖凝胶电泳结果显示:ATRA组为呈一定间隔的DNA梯形条带,MMC组呈现弥漫的片状图谱,无明显的条带。结论:ATRA可有效抑制体外培养的HTCFs的增殖,但与MMC的作用方式不同。MMC主要造成细胞坏死,而ATRA则主要诱导细胞凋亡,无明显细胞毒作用。Objective： To study the effect of all trans-retinoic acid （ATRA） on the proliferation and apoptosis of human Tenon＇s capsule fibroblasts（HTCFs） in vitro. Methods： Cell cycle distribution, the apoptotic rate and the expression of bcl-2 were determined by flow cytometry. Cell DNA was determined by agarose gel electrophoresis. Mitomycin C was used as a positive control. Results： Cell cycle distribution of HTCFs showed that cells of the G0/G1 phase increased and of the S phase decreased after treatment with ATRA and MMC. Cellular apoptosis was decreased by ATRA at a lower dose （ 1 × 10^-9 mol/L）, and the apoptotic rate was significantly increased in a dose-dependent manner. Expression of bcl-2 was significantly decreased by ATRA. A ＂ladder＂ strand of DNA was produced from the ATRA groups by DNA agarose gel electrophoresis. Condusion： ATRA can significantly inhibit the proliferation of HTCFs and induce the apoptosis of HTCFs without apparent cytotoxicity.

关 键 词：维甲酸 成纤维细胞 Tenon囊 细胞增殖 细胞凋亡 

分 类 号：R775.5[医药卫生—眼科]