单抗介导的血凝试验对H_9N_2亚型禽流感病毒的研究  

Study on H_9N_2 Subtype Avian Influenza Virus by Monoclonal-antibody-Haemoglutination Inhibition Test

作　　者：王新卫[1] 李建华[1] 刘守川[1] 杨大光[1] 王泽霖[1] 

机构地区：[1]河南农业大学牧医工程学院,河南郑州450002

出　　处：《长江大学学报（自科版）（中旬）》2007年第1期23-25,共3页Journal of Yangtze University(Nature Science Edition)

摘　　要：利用4株鼠源抗H9N2禽流感病毒血凝素单克隆抗体与标准H9N2禽流感多克隆抗体,通过血液凝集抑制试验(Haemoglutination inhibition test,HI)分别对25株H9N2禽流感病毒进行了检测,发现4株单克隆抗体均对其中的23株H9N2禽流感病毒反应,其最高HI效价分别为:A单抗12log2,B单抗12log2,C单抗12log2,D单抗12log2;最低分别为:A单抗3log2,B单抗9log2,C单抗4log2,D单抗6log2,而对其中的2株H9N2禽流感病毒没有HI反应,其HI价均为0;然而,标准多抗对25株H9N2禽流感病毒均产生HI反应。检测结果表明2株H9N2AIV毒株血凝素蛋白出现了抗原漂变。25 strains H9N2 AIV were assayed through haemoglutination inhibition test(HI)by making use of 4 McAb(monoclonal antibody,McAb)to HA protein of H9N2-AIV and positive standard serum(anti-H9N2-AIV)respectively.The results showed that 23 of 25 strains H9N2 AIV reacted to the 4 McAb.The highest HI titer were 1∶212 for A McAb,1∶212 for B McAb,1∶212 for C McAb and 1∶212 for D McAb,respectively.The lowest titer were 1∶23 for A McAb,1∶29 for B McAb,1∶24 for C McAb and 1∶26 for D McAb respectively.However,2 of 25 strains H9N2 AIV had no HI reaction to all the 4 McAb,and their HI titers were 0.At the same time,the standard serum to H9N2 AIV could react to all the 25 strains H9N2 AIV.This indicated that 2 strains 17 and 18 H9N2 AIV strains had antigen variation in their HA.

关 键 词：单抗 H9N2 禽流感病毒 血凝抑制试验 

分 类 号：S852.4[农业科学—基础兽医学]