槐耳浸膏对骨髓瘤细胞增殖和凋亡的影响  

Impact of Huaierjingao on the proliferation and apoptosis of myoloma cell induced by PS-T

作　　者：钱红兰[1] 胡倩[1] 胡旭东[1] 陈怡[1] 孙岚[1] 梁彬[1] 叶海格[1] 

机构地区：[1]温州医学院附属第一医院血液科,浙江温州325000

出　　处：《中国热带医学》2007年第6期889-890,共2页China Tropical Medicine

基　　金：浙江省温州市科技局项目资助(20060136)

摘　　要：目的探讨槐耳浸膏对骨髓瘤细胞株PRMI8226细胞增殖和凋亡的影响。方法取PRMI8226细胞与1.5mg/ml、3mg/ml、5mg/ml的不同浓度槐耳浸膏在体外孵育,分别在24h、36h、和48h时用CCK-8法检测细胞增殖抑制率,并用流式细胞术(FCM)检测早期细胞凋亡情况。结果槐耳浸膏可抑制PRMI8226细胞增殖,不同浓度的槐耳浸膏对多发性骨髓瘤细胞增殖的抑制效果不同,以5mg/ml抑制增殖效果最好。且浓度为5.0mg/ml的金克诱导多发性骨髓瘤细胞48h抑制率达到高峰84%。FCM检测表明槐耳浸膏可诱导PRMI8226细胞早期凋亡,用5.0mg/ml槐耳浸膏处理48h后,可引起25.9%的PRMI8226细胞凋亡。这两种作用均随槐耳浸膏浓度和作用时间的延长而增强。结论槐耳浸膏可在体外抑制PRMI8226细胞增殖,并促进其凋亡。Objective To investigate the effects of Huaierjingao （PS - T） on proliferation and apeptosis of myelorna cell llne PRMI8226 in vitro. Methods PRMI8226 eells were cultured with different concentrations of PS - T （1.5, 3.0 and 5. Omg/ml）. The inhibition of proliferation was measured by CCK - 8 assay 24, 36 and 48 hours after culture and cell apeptosis was evaluated by flow eytometry. Results PS - T inhibited the growth of PRMI8226 cells in concentration and time - dependent manner. Flow eytometry analysis showed that PS - T induced apeptosis of PRMI8226 cells, and the percentage of apoptotie cells increased as the concentration increased and time elongated. Conclusion PS - T can inhibit proliferation of PRMI8226 cells and promote its apeptosis.

关 键 词：多发性骨髓瘤 槐耳浸膏 细胞增殖 细胞凋亡 

分 类 号：R733.3[医药卫生—肿瘤]