高甘油三酯对不同方法测定HDL-C的影响研究  

Observation on the effect of high concentration of serum triglyceride on the detection of high density ipoprotein cholesterol by various mehtods

作　　者：赖雄 余岸松[2] 

机构地区：[1]湛江市第二人民医院检验科,广东湛江524003 [2]广东海洋大学医院,广东湛江524088

出　　处：《中国热带医学》2007年第6期994-995,共2页China Tropical Medicine

摘　　要：目的研究不同浓度血清甘油三酯（TG）对高密度脂蛋白胆固醇（HDL-C）测定的影响,寻找更适合基层实验室测定HDL-C的方法。方法确定TG〈1.69mmol/L30例及高血脂标本103例,采用全自动生化分析仪检测TG及HDL-C,并稀释高甘油三酯标本,采用直接法（过氧化氢酶清除法）及沉淀法进一步检测HDL-C,2种方法检测结果进行比较。结果在TG〈1.69mmol/及在1.69-4.00mmol/L时,2种方法测定HDL-C结果之间无差异（P〉0.05）;在TG〉4.01mmol/L时,2种方法之间差异有显著性（P〈0.05）。稀释标本检测,在TG〉6.00mmol/L时,2种方法之间HDL-C差异有显著性（P〈0.05）;同一方法检测标本稀释前后HDL-C结果比较,在TG各浓度值直接法均无差异,在TG〉4.00mmol/L时,沉淀法有差异。结论检测高TG标本HDL-C,直接法和沉淀法之间有显著差异,有条件的实验室应选择采用过氧化氢酶清除原理的直接法检测高TG标本。如采用沉淀法,应注意高TG对测定结果的影响。Objective To investiate the effect of different concentrations of serum triglyceride （TG） on the determination of high - density lipoprotein cholesterol （ HDL - C） and look for a mehtods suitable for determinatio nor HDL - C at grass - roots laboratory. Methods TG、HDL - C in serum were determined by autobioehemieal analyzer, and dilute serum specimen of high concentrations triglyceride specimen, and HDL - C was further detected by the methods of direct and precipitation, and the results of HDL - C were compared. Results There no difference was observed in determination of HDL- C between the 2 groups and the concentrations of serum TG was in the ranges of 1.69 - 4.00nnaoL/L. there significant difference was observed in determination of HDL - C as TG concentration was higher than 4.0mmol/L （P 〈 0.05）. In determiantion of HDL - C befor and afer dilution of serum specimen significant differences were noticed as TC concentrations were higher than 6.00mmol/L; when HDL- C of diluted serum specimen was determined by identical methods, there was no difference among all with the methods of direct, there was difference as TG was higher than 4.01mmot/L with precipitation. Conclusion Significant differences in indetermination of concentration ofHDL - C with high TG level by precipitation and directly automatic methods have been observed, suggesting that HDL- C in the sera with high TC; level should be determined by catalase HDL - C assay of directly automatic determination method.

关 键 词：甘油三酯 高密度脂蛋白胆固醇 

分 类 号：R-33[医药卫生]